# Analysis FAQ for Illumina DNA PCR Free libraries

What **is the recommended analysis workflow?**

* Illumina DNA PCR-Free data generated from the NovaSeq 6000 can be analyzed, visualized, and interpreted with the TruSight Software Suite.
* Illumina DNA PCR-Free data can also be evaluated utilizing the Illumina DRAGEN Germline pipeline v3.4+.
* Alternative pipelines can be used to analyze data; however, different analyses may provide slightly different results.

**What are the adapter trimming sequence(s) for this library?**

* Trim both Read 1 and Read 2 with: CTGTCTCTTATACACATCT+ATGTGTATAAGAGACA

\*\*Are there Illumina DNA PCR-Free precision and recall statistics for NA12878 using the latest DRAGEN pipeline and how do these numbers change with decreased coverage? How do the results compare to TruSeq DNA PCR-Free?\*\*See BaseSpace [Demo Data](https://basespace.illumina.com/datacentral) sets for Illumina DNA PCR-Free.\
**Are example datasets available?**

See BaseSpace [Demo Data](https://basespace.illumina.com/datacentral) sets for Illumina DNA PCR-Free.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3440), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003440%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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