Thumbnail image information on the NextSeq 500/550

On the NextSeq 500/550, thumbnail images are disabled by default to prevent Real Time Analysis (RTA) from running slowly.

• Enabling thumbnail images can aid in troubleshooting.

Thumbnail images location: and nomenclature

• File Path: D:\Illumina\ NextSeq Control Software Temp\

• Temp[Run_Folder]\Thumbnail_Images\L00[4]\C[25.1] (Lane 4, Cycle 25, tiles 01)

Thumbnail images nomenclature:

• [Run_Folder]\Thumbnail_Images\L00[X]\C[Y.1] ]

  • X refers to the lane number

  • Y refers to the cycle number.

• Saves thumbnail images only for tiles 01, 06, and 12.

• Flow cell

  • 4 Lanes

  • 2 Surfaces (1 = top, 2 = bottom)

• Swaths

  • Mid Output flow cell = 1 swath

  • High Output flow cell = 3 swaths

  • 6 sections (six cameras)

• Tiles represented in two digits

  • Mid Output flow cell: 144 tiles (4 lanes x 1 swath x 3 sections x 12 tiles)

  • High Output flow cell: 432 tiles (4 lanes x 3 swaths x 3 sections x 12 tiles)

  • Both flow cell tiles are numbered 01--12

• Thumbnail file naming format: s_[lane][tile numbering][channel].jpg

• Tile numbering format: [Surface][Swath][Section][Tile]

  • Example:

    • s_2_21206_green.jpg

      • lane 2

      • bottom surface

      • swath 1

      • section 2

      • tile 06

      • green channel

References:

• Two-Channel SBS Technology

• NextSeq 500/500 Sequencing Systems Product Documentation (section Flow Cell Tiles in Sequencing Output files)

• For further information, see for Knowledge Base article How to turn on thumbnails for troubleshooting on the NextSeq 500/550.

For any feedback or questions regarding this article (Illumina Knowledge Article #3084), contact Illumina Technical Support [e-mail].