# Does library loading differ between MiSeq Micro and Nano flow cells?

Libraries will be denatured and diluted to the same final volume of 600 µL regardless of MiSeq flow cell is used.

If a library loading concentration has been optimized using MiSeq v2 reagents with a standard flow cell, the same loading concentration should be used with a Micro or Nano flow cell. If a library loading concentration has been optimized with MiSeq v3 reagents, the loading concentration may need to be slightly lowered when using a Micro or Nano (or standard output v2) flow cell due to the lower cluster density supported with MiSeq v2 reagents.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #5606), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000005606%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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