How to Disable Cloud Login Feature on the MiSeq i100 and NovaSeq X Series

Background

The MiSeq i100 and NovaSeq X Series instruments require users login to the Control Software using either a Local or Cloud login. If the instrument is not connected to the internet and the Cloud login is not required, use the following steps to disable the Cloud login feature and only use the Local login.

The Cloud login should only be disabled if the instrument is completely disconnected from an internet connection. This procedure disables all Cloud upload features including both Proactive and BaseSpace features. For more information on Proactive, see the following resources:

Illumina Proactive - Instrument Services

Proactive connectivity guide for Miseqâ„¢ i100 Series

Proactive connectivity guide for NovaSeq X Series

Resolution Actions

Disabling Cloud Login

1. Ensure the instrument is idle and there are no Active sequencing Runs or DRAGEN Analyses.

2. Login to the instrument as an Administrator account.

3. Access the Settings Menu:

   1. On the MiSeq i100 Series Control Software Home screen, select the dropdown menu in the top-left corner, then select Settings.

   2. On the NovaSeq X Series Control Software Home screen, select the instrument icon, then select Settings.

4. Select Cloud Settings.

5. De-select all checkboxes under Run settings and Illumina Proactive.

6. Remove any entries in the Private domain name field.

7. Within the Hosting Location dropdown menu, select the blank entry above the BSSH USA (N. Virginia) selection (Figure 1).

   1. The Hosting location selection should be empty after selection (Figure 2).

8. Select Save.

If the Cloud Settings menu presents the message "An unexpected error has occurred. Details: Internal Server Error," see the following steps.

1. Navigate to the Cloud Settings menu.

2. Confirm that all checkboxes are de-selected and the Hosting Location is blank.

3. Select Save, then wait 2 minutes. It is expected to display a 'loading circle' that does not resolve.

4. After 2 minutes, select the X in the top-right corner of the Cloud Settings UI.

5. Navigate back to the Home screen, then select the dropdown menu in the top-left corner, and then sign out of the Admin account.

6. Click the screen to initiate Control Software login and confirm if Local Login presents as the default option.

Figure 1: Highlighting the empty Hosting location selection within the dropdown menu.

Figure 2: Highlighting the empty Hosting location selection.

Re-enabling Cloud Login

1. Ensure the instrument is idle and there are no Active sequencing Runs or DRAGEN Analyses.

2. Login to the instrument as an Administrator account.

3. Access the Settings Menu:

   1. On the MiSeq i100 Series Control Software Home screen, select the dropdown menu in the top-left corner, then select Settings.

   2. On the NovaSeq X Series Control Software Home screen, select the instrument icon, then select Settings.

4. Select Cloud Settings.

5. Select the appropriate regional Hosting Location using the dropdown menu.

6. For Enterprise users, input the Private domain name in the free text field.

7. Select the desired checkboxes under Run settings and Illumina Proactive.

   1. Illumina Proactive - Uploads hardware and run performance data to Illumina Support for troubleshooting purposes.

   2. Cloud run monitoring - Uploads run metrics to BaseSpace for user run monitoring.

   3. Cloud run storage - Uploads run metrics and data to BaseSpace, required for Cloud Analysis.

8. Select Test Configuration and confirm all required URLs are reachable.

   1. Consult the local IT team to add the affected URLs to local network security allow list if any URLs are not reachable.

9. If Test Configuration is PASS, select Save.

For any feedback or questions regarding this article (Illumina Knowledge Article #9462), contact Illumina Technical Support [e-mail].