> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003093.md).

# How to obtain a NextSeq 1000/2000 v2 sample sheet?

A v2 sample sheet can be obtained from one of the following options.

1. A v2 sample sheet can be created in BaseSpace using Run Planning, with the following steps.

* In BaseSpace Sequence Hub, select the **Runs** tab > **New Run** > **Run Planning** > **Local Mode** > **Export Sample Sheet**.
* For this option, all sample and index information is entered in Run Planning, and is included in the exported v2 sample sheet.

2. A v2 Sample sheet template is available for download [here](https://help.connected.illumina.com/run-set-up/overview).

* * To manually fill the template, use a text editor program to alter the sections according to your chosen instrument or analysis. *Using a spreadsheet editor can cause errors with sample sheet validation and analysis.*\
    See resources below for more information:\
    1\. [Sample Sheet Structure](https://help.connected.illumina.com/run-set-up/overview/sample-sheet-structure)\
    2\. [Instrument Settings](https://help.connected.illumina.com/run-set-up/overview/instrument-settings)\
    3\. [Cloud Analysis Auto-launch Settings](https://help.connected.illumina.com/run-set-up/overview/auto-launch-settings)\
    4\. [Index Orientation Guide](https://help.connected.illumina.com/run-set-up/overview/index-orientation-guide)\
    5\. [Resources](https://help.connected.illumina.com/run-set-up/overview/resources)

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                        |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #3093), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003093%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter:

```
GET https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003093.md?ask=<question>&goal=<endgoal>
```

`ask` is the immediate question: it should be specific, self-contained, and written in natural language.
`goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal.

The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.
