How to obtain a NextSeq 1000/2000 v2 sample sheet?

A v2 sample sheet can be obtained from one of the following options.

1. A v2 sample sheet can be created in BaseSpace using Run Planning, with the following steps.

• In BaseSpace Sequence Hub, select the Runs tab > New Run > Run Planning > Local Mode > Export Sample Sheet.

• For this option, all sample and index information is entered in Run Planning, and is included in the exported v2 sample sheet.

1. A v2 Sample sheet template is available for download here.

• • To manually fill the template, use a text editor program to alter the sections according to your chosen instrument or analysis. Using a spreadsheet editor can cause errors with sample sheet validation and analysis. See resources below for more information: 1. Sample Sheet Structure 2. Instrument Settings 3. Cloud Analysis Auto-launch Settings 4. Index Orientation Guide 5. Resources

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