Library denaturation and compatibility for the iSeq 100 system
Libraries loaded onto the iSeq 100 system must be double-stranded DNA, as libraries are automatically denatured into single strands onboard the instrument. Bead-normalized libraries are single-stranded and are not compatible with the iSeq 100.
The following Illumina library prep protocols include an optional bead normalization procedure:
Important Note: The lone exception to the requirement of double-stranded DNA is the Illumina DNA PCR-Free library preparation workflow. Although this workflow yield single-stranded DNA, it has been validated for use on the iSeq 100 when utilizing iSeq Control Software v3.0. This is due to the requirement of VP10 and VP14 as primers for Read 1 and Index Read 2, respectively. These are sold separately (catalog # 20041797) and must be loaded as custom primers on board the instrument, hence the requirement for updating to iSeq Control Software v3.0.
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