Computer Hardware Specifications for NovaSeq 6000

The NovaSeq 6000 includes two computer systems, the Compute Engine (CE) and the Control Computer (SBC). The SBC is a Windows-based computer mapped to the C and D drive, whereas the CE is a Linux-based computer mapped to the Z:\ drive and stores .cbcl data before copying to the network drive.

Specifications for each drive on NovaSeq6000:

Single-Board Computer (SBC): total available space is 250 GB (partitioned into C:\ and D:\ drives).

• C drive - 174 GB available

  • Windows 10 Version: Windows 10 Enterprise LTSC.

  • Contains Windows and Maintenance Logs.

  • Contains information from NovaSeq Control Software (NVCS), NovaSeq Service Software (NVSS), and Universal Copy Service (UCS).

  • Responsible for USB and Ethernet port management.

  • Requires the following amount of space to start a run: - SP run: 5 GB - S1 run: 20 GB - S2 run: 20 GB - S4 run: 40 GB

• D drive - 64 GB

  • Stores instrument performance data.

  • The instrument will manage the data on this drive automatically in most cases.

Compute Engine (CE) drive (Linux): Early models have 2.85 TB; Current production models have 3.14 TB of space available.

• Runs Real Time Analysis v3 (RTA3) and handles data processing.

• Will be mapped as the Z:\ drive.

• Has a single folder, 'outputfolder' that will have the run data.

• Requires the following amount of space per cycle to start run:

  • S1 run: 1.35 GB

  • S2 run: 5.4 GB

  • S4 run: 4.3 GB

• Runs must be backed up to a network location (either local network or BaseSpace Sequence Hub).

• Disc space is checked at the start of each run (clear space using Process Management only).

• Requires 1 GB ethernet connection.

• Recommended using CAT-6 shielded cable.

• 200 MB/s upload speed for Base Space Sequence Hub (BSSH) (5 MB/s for Proactive data).

• Dedicated internal IP connections. For more information, refer to NovaSeq 6000 Site Prep Guide.

• A network card capable of up to 1 Gbps speed is required.

• Information about the power cord:

  • NovaSeq 6000 instrument ships with UPS (Uninterruptible Power Supply (UPS) Models by Instrument and Troubleshooting).

  • User socket to the uninterruptible power supply (UPS) uses power cord: NEMA-L6-20P to C19 This is a US/ Canada/ South America (not Brazil) cord & is 8 ft (2.5 m) long.

  • Other regions have different cords, but all are typically 2.5 meters long.

  • UPS backup outlet to NovaSeq 6000 uses power cord: C20 to C19. This is used on almost all UPS (not Japan) and is 2 meters (6 ft) long.

  • UPS cord connects to electrical supply with locking connection plug (type L6-20P), which needs an L6-20R socket.

The NovaSeq 6000 computer name can be changed with the following steps.

• File Explorer, right-click on "This PC" and select Properties.

• In properties, select "Change settings" for the computer name, domain, and workgroup settings.

For any feedback or questions regarding this article (Illumina Knowledge Article #2428), contact Illumina Technical Support [e-mail].