How to troubleshoot NovaSeq 6000 “A component failed during sequencing” error during a Cloud Outage

Background

When starting a NovaSeq 6000 Sequencing run and BaseSpace/Proactive are unavailable, the error message "Component failed during sequencing" can be observed shortly after starting the sequencing run.

Troubleshooting steps

If there is no sequencing run in progress on the other side, evaluate the following to see if the same reagents can be reloaded.

1. Confirm that the reagents are unpierced and no run folder has been generated under C:\ProgramData*\Illumina\NovaSeq\NovaSeqTemp.

• The ProgramData folder is Hidden by default. If this folder is not present, select the View tab in File Explorer and then select the checkbox next to Hidden Items.

2. Review status.illumina.com to see if BaseSpace is currently experiencing a service incident in the relevant region.

If either 1 or 2 are not applicable, refer to the Knowledge Base article How to troubleshoot “A component failed during sequencing” error messages on the NovaSeq 6000 for more information.

Resolution actions

If there is a BaseSpace incident that is affecting NovaSeq 6000 run setup, see the following steps as a temporary mitigation until the incident has been resolved.

Important: Illumina recommends starting Xp flowcells within 30 minutes of loading the samples onto the flow cell. If 30 minutes have already elapsed from loading the Xp samples onto the flow cell, contact Technical Support for further assistance.

1. From the NovaSeq 6000 Control Software home screen, select the dropdown menu in the top-left corner, then select Settings.

2. On the Settings page, de-select any checkboxes for BaseSpace Sequencing Hub or Illumina Cloud options.

• When BaseSpace Sequence Hub is not enabled, an Output Folder is required.

3. Additionally, de-select the checkbox next to 'Send Instrument Performance Data to Illumina'

4. Select Save.

5. Restart the sequencing run setup using the same consumables from the previous run attempt.

6. After the BaseSpace incident has been resolved, these Cloud settings can be reverted back to previous selections to return instrument to the previous workflow.

   

For any feedback or questions regarding this article (Illumina Knowledge Article #9026), contact Illumina Technical Support [e-mail].