# What effect does prolonged flow cell staging time have on clustering for the NovaSeq 6000? NovaSeq 6000 Standard Workflow: Prolonged staging time between denaturation and the start of the sequencing run can cause denatured libraries to reanneal, leading to reduced clustering efficiency. NovaSeq 6000 Xp Workflow: Prolonged flow cell staging time between ExAmp master mix combination with pooled libraries and the start of the sequencing run can cause increased % occupancy and lower % pass filter. As a general rule, the maximum recommended flow cell staging time is 30 minutes. ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-90a66f2cea56d2893da18286d28d49d5a98f455c%2Fimage1.jpg?alt=media\&token=b4c1fc5a-7868-48d2-90bd-aa634c5eb798) For more information, see the technical note, [The NovaSeq Xp workflow provides flexibility and control without sacrificing data quality or yield](https://support.illumina.com/content/dam/illumina-marketing/documents/products/technotes/novaseq-xp-workflow-tech-note-770-2018-014.pdf). \ \ \
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