# What effect does prolonged flow cell staging time have on clustering for the NovaSeq 6000?
NovaSeq 6000 Standard Workflow:
Prolonged staging time between denaturation and the start of the sequencing run can cause denatured libraries to reanneal, leading to reduced clustering efficiency.
NovaSeq 6000 Xp Workflow:
Prolonged flow cell staging time between ExAmp master mix combination with pooled libraries and the start of the sequencing run can cause increased % occupancy and lower % pass filter. As a general rule, the maximum recommended flow cell staging time is 30 minutes.
For more information, see the technical note, [The NovaSeq Xp workflow provides flexibility and control without sacrificing data quality or yield](https://support.illumina.com/content/dam/illumina-marketing/documents/products/technotes/novaseq-xp-workflow-tech-note-770-2018-014.pdf).
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