How to use the Auxiliary File for Infinium BeadChips with Enhanced PGx Content
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The Auxiliary file identifies markers that are used by the following DRAGEN Array Analysis types:
DRAGEN Array - PGx - CNV calling
DRAGEN Array - PGx - Star allele annotation
The tab-delimited text file can be imported into GenomeStudio and used to review and optimize PGx variant GenTrain cluster positions. The Auxiliary file is provided for each PGx Array product and is available to download from the page or the respective BeadChip Product Files page.
Create a GenomeStudio 2.0 genotyping project using the standard PGx product files provided by Illumina.
Download the BPM manifest and EGT cluster file from the BeadChip Product Files page:
For more information on creating a GenomeStudio genotyping project, see the and .
Download the Auxiliary file and extract the .txt file which contains information for several thousand probes.
BeadChip
Number of probes
Global Diversity Array v1.0 + ePGx (GDA + ePGx)
5811
Global Screening Array v4.0 + ePGx (GSA v4 + ePGx)
7333
Global Clinical Research Array v1.0 + ePGx (GCRA + ePGx)
7442
Import the Auxiliary file into the GenomeStudio Full Data Table or SNP Table.
Repeat Steps 3-4 for the other table. For example, if columns were imported into Full Data Table first, repeat for SNP Table.
Column Name
Explanation
probe_in_map
A value of 1 means the probe is used for PGx CNV calling and star allele annotation. Filter by this column to display the probes used for PGx CNV calling and star allele annotation.
combined_alleles
Values
Simple variant (begins with rs)
Star allele haplotype
Alleles are delimited by trailing semicolon. Always include trailing semicolon if using filter or using Find function in GenomeStudio. This will ensure search is specific to intended query (For example, if searching for *11 type *11; to ensure no unintended results for other star alleles)
gene
combined_hgvs
Examples:
Reference sequence for genomic (nucleotide): NC_ is a genomic reference sequence based on a chromosome
Types of variants include substitution, deletion, duplication, insertion, deletion/insertion
rsId
The rsIDs are derived from public pharmacogenomics databases.
Select 'Import columns into the table' icon.
The 'Import' window appears. Select 'Column Import' and browse to the location of the 'Import.txt' file.
Five new columns will appear in the table: rsId, combined_hgvs, combined_alleles, gene, and probe_in_map.
Select 'Filter rows' icon.
Filter by column 'probe_in_map' = 1 and click 'OK'.
The table will display a filtered list of several thousand rows which are used for PGx CNV calling and star allele annotation.
Provides associated Gene name. Use this column to filter for genes of interest. Review for more information.
Human Genome Variation Society (HGVS) nomenclature is used to denote variants. Every probe has a HGVS tag which includes the variant information. Refer to this resource on nomenclature:
For additional information, refer to the .
For any feedback or questions regarding this article (Illumina Knowledge Article #8505), contact Illumina Technical Support .