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MiSeq Flow cell compatibility for v2 and v3 reagent kits

The standard MiSeq flow cells are identical in MiSeq v2 and MiSeq v3 sequencing kits.* If needed, a flow cell from a MiSeq v2 kit can be used with a MiSeq v3 cartridge, or a flow cell from a MiSeq v3 kit can be used with a MiSeq v2 reagent cartridge.
  • Note: Micro and Nano flow cells are only compatible with v2 reagent cartridges.
For any feedback or questions regarding this article (Illumina Knowledge Article #2538), contact Illumina Technical Support [email protected].
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