# Illumina测序系统废液中甲酰胺的终浓度是多少？
该技术文档提供的信息，能够帮助您计算Illumina测序反应废液中甲酰胺的终浓度。
每个测序反应废液中甲酰胺的浓度会基于测序平台和测序长度而有所差异。您可以根据下表的说明，测量废液的总体积，并计算甲酰胺的终浓度。
\*由于iSeq100试剂槽不能打开，因此它是以固体废弃物处理。
\*\*如果剩余甲酰胺没有排入废液中，基于不同测序长度甲酰胺的浓度范围为0.35-1.5%
**NovaSeq X/X Plus**
NovaSeq X/X Plus的废液中甲酰胺浓度取决于所执行的Reads和Index读取的次数。
有关NovaSeq X/X Plus废液的更多信息，请参见[Handling and Disposal Recommendations for Used Reagents on the NovaSeq X/X Plus](https://knowledge.illumina.com/instrumentation/novaseq-x-x-plus/instrumentation-novaseq-x-x-plus-faq-list/000008392).
**MiSeq i100 / i100 Plus**
试剂卡盒不含甲酰胺，可以像PCR产品废弃物一样丢弃。
请穿戴合适的个人保护装备安全处理甲酰胺试剂。在不同地区，根据废液中甲酰胺和其它有害成分，对废液的处理也有所差异。请联系当地的环境卫生和安全（EH\&S）办公室，确定对有害物质的界定以及如何处理容器和未使用的有害物质，以符合环境安全。如果需要Illumina试剂中化学成分的更多信息，请参考Illumina支持页面中的[安全数据表（SDS）](https://support.illumina.com/sds.html)。
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