# 如何使MiSeq Reporter生成Index Reads 的FASTQ文件

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在默认情况下，MiSeq Reporter不会创建样本Index的FASTQ文件。如果用户需要创建该文件，请按以下步骤配置MiSeq Reporter：

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***请注意**：用户修改好配置后，需以管理员身份重启MiSeq Reporter service后才能生效。如果没有管理员权限，可以通过重启电脑的方式来重启MiSeq Reporter service。*

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1. 打开**C:\Illumina\MiSeqReporter**文件夹
2. 备份**MiSeq Reporter.exe.config**文件
3. 使用Notepad编辑原始**MiSeq Reporter.exe.config**文件
4. 在文件的顶部（**\<appSettings>** 部分）添加以下字段：**\<add key="CreateFastqForIndexReads" value="1" />**
5. 保存文件
6. 重启MiSeq Reporter service
   1. 在键盘上同时按住Ctrl-Alt-Delete按键后，在新弹出的对话框中选择启动任务管理器Windows Task Manager
   2. 选择**services**标签窗口
   3. 在services列表中找到**MiSeqReporter**选项
   4. 选择停止服务：右击**MiSeqReporter**选项，在新弹出对话框选择**停止**服务
   5. 选择重新启动服务：右击**MiSeqReporter**选项，在新弹出对话框选择**启动**服务。

修改该参数后，MiSeq Reporter的所有分析都会为每个样本产生对应的index read FASTQ文件。如果想要停止输出该文件，&#x5C06;**\<add key="CreateFastqForIndexReads" value="1" />** 中的value="1"改为value="0"后保存然后按照上述第6步重启MiSeq Reporter service即可。

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #7352), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000007352%20-%20Software%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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