> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/software/on-instrument-analysis-software/software-on-instrument-analysis-software-reference_material-list/000003678.md).
# How to Specify Adapters for Trimming in Local Run Manager
**How to Specify Adapters for Trimming in Local Run Manager**
1. From the Module-Specific Settings section of the Create Run screen, select "Show Advanced Settings".
2. Select "+ Add custom setting."
3. In the custom setting field, enter the setting name, in this case: "Adapter."
4. In the setting value field, enter the setting value. In order to specify 2 different adapter sequences for Read 1, type in the two sequences separated with a plus (+) sign. These will be assessed as separate adapters.\
5. If a Read 2 adapter trimming sequence is also needed, repeat steps 2 - 4, but use the setting name "AdapterRead2." In order to specify 2 different adapter sequences for Read 2, type in the two sequences separated with a plus (+) sign. These will be assessed as separate adapters. 
6. To remove a setting, select the "X".
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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3678), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003678%20-%20Software%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |
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