# Information and protocol for the Illumina Fungal Metagenomics Sequencing workflow

The Illumina Fungal Metagenomics Sequencing demonstrated protocol is a two-step PCR workflow that amplifies the ITS1 region from samples for metabarcoding studies.

The protocol is available on the page, [here](https://support.illumina.com/downloads/fungal-metagenomic-sequencing-demonstrated-protocol-1000000064940.html).

Compatible index primer kits are listed on the Nextera XT product page, [here](https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/nextera-xt-dna.html), under the Index Adapters heading. (**Note**: the Illumina Fungal Metagenomics protocol does not use the Nextera XT library preparation kit, this link is only to reference compatible index primer kits.)

For more information, see the application note: [Fungal sequencing and classification with the](https://www.illumina.com/content/dam/illumina-marketing/documents/products/appnotes/its-metagenomics-app-note-1270-2018-001-web.pdf) [ITS Metagenomics Protocol](https://www.illumina.com/content/dam/illumina-marketing/documents/products/appnotes/its-metagenomics-app-note-1270-2018-001-web.pdf).

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #5773), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000005773%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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