Sequencing primer compatibility of Illumina libraries and library types for the iSeq 100

Illumina offers a wide array of library preparation kits and sequencing platforms for different applications, each with its own coverage and indexing considerations. The table below lists the Illumina sequencing primers and library preparation kits compatible with the iSeq 100 platform based on the required data output\*, read length, and the indexing capabilities of each kit.

Note: Most Illumina libraries can be sequenced with all Illumina sequencing platforms. If a specific Illumina library is not listed below, contact Illumina Tech Support to discuss special considerations.

Refer to the Sequence Coverage Calculator and Library Prep and Array Kit Selector resources for additional information.

Table 1. Compatible sequencing primers and recommended library types for the iSeq 100 sequencing platform.

\*The iSeq 100 sequencing platform has a maximum data output of 1.2 Gb.

For any feedback or questions regarding this article (Illumina Knowledge Article #1669), contact Illumina Technical Support [e-mail].