# Troubleshooting Insufficient Space on External Storage on the MiSeq i100 Series Instruments. **Background** The MiSeq i100 Series requires External Storage location for transferring data off the instrument during Sequencing Runs and Secondary Analysis. Insufficient storage space to complete the data transfer will typically result in the transfer automatically cancelling and can also impact Secondary Analysis. This issue will typically present as Analysis Failed under the Run Details page, indicating Error occurred during analysis external transfer. Under the **File transfer status** header, the Sequencing data transfer will typically show as `Cancelled` and the Secondary analysis data transfer will show as `Failed`. ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-32f2e4b92a05b3074063c3653035e276e7e74cc5%2Fimage1.png?alt=media) To confirm the issue, select the **...** ellipses next to **Sequencing data transfer** and then select **View details**. Review the **File transfer error(s)** section for `no space left on device` messages: ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-aea6e54e27f73b7e9fd73dec7dc22019b8f635ee%2Fimage2.png?alt=media) **Resolution Actions** ### *Resolving Data Transfer Issues* #### #### For Networked External Storage: 1. Use a separate computer to connect to the network storage, then delete or archive old sequencing runs and data to clear space for the Sequencing Data and Secondary Analysis Data on the networked External Storage. 1. See [Estimating Disk Space Utilization on the MiSeq i100 Series](https://knowledge.illumina.com/instrumentation/miseq-i100-series/instrumentation-miseq-i100-series-reference_material-list/000009356) for additional details. 2. On the Control Software Home screen, select the **main menu** in the top-left corner, then select **Runs**. 3. Within the Runs user interface (UI), select the **Completed** tab. 4. Under the Run Name column, select the **sequencing run** to open the Run Details UI. 5. In the Run Details UI, select the **Show More** dropdown next to **Run Status**. 6. Select **Restart file transfer**. 7. Use the **Copy output location** dropdown to select the desired location from the list of configured output folder locations. 8. Select **Restart**. #### For USB Hard Drive External Storage: 1. From the Control Software Home screen, select the **main menu** in the top-left corner and then select **Settings**. 2. Select **External Storage**. 3. Locate the configured **USB External Storage** in the list under **Network Storage** header, then select **Eject** to safely remove the USB External Storage. 4. Unplug the USB drive and transfer the drive to another computer. 5. Once connected to another computer, delete or archive old sequencing runs and data to clear enough space for the Sequencing Data and Secondary Analysis Data on the USB drive. 1. See [Estimating Disk Space Utilization on the MiSeq i100 Series](https://knowledge.illumina.com/instrumentation/miseq-i100-series/instrumentation-miseq-i100-series-reference_material-list/000009356) for additional details. 6. Safely eject the USB drive, then transfer the drive to the MiSeq i100 instrument and plug into the **USB 3.0** port on the left side. 7. Follow the instructions under [External Storage - Add a USB Drive](https://support-docs.illumina.com/IN/MiSeqi100Series/Content/IN/MiSeqi100/SysAddUSB.htm) documentation to add the USB drive as an External Storage device, then specify an output folder on the drive. 8. From the Control Software Home screen, select the **main menu** in the top-left corner, then select **Runs**. 9. Within the Runs user interface (UI), select the **Completed** tab. 10. Under the Run Name column, select the **sequencing run** to open the **Run Details** UI. 11. In the Run Details UI, select the **Show More** dropdown next to **Run Status**. 12. Select **Restart file transfer**. 13. Use the **Copy output location** dropdown to select the configured USB External Storage location from the list of configured output folder locations. 14. Select **Restart**. ### *Resolving Secondary Analysis Failures* Issues with data copy to configured External Storage can frequently cause Secondary Analysis to not complete as expected. See the following steps below to requeue Secondary Analysis after clearing space on the configured External Storage. 1. Allow the Sequencing data transfer to complete copying to External Storage if possible. 2. Once complete, see section *Resolving Data Transfer* *Issues* to clear space on the configured External Storage. 3. From the Control Software **Home** screen, select the dropdown menu in the top-left corner, then select **Runs**. 4. Within the Runs user interface (UI), select the **Completed** tab. 5. Under the Run Name column, select the **sequencing run** to open the **Run Details** UI. 6. Scroll down to the **Secondary Analysis** header, then select **Requeue Analysis**. 7. Follow the prompts to requeue the analysis using the **Run Planning** tool or from a **sample sheet**. 8. Use the dropdown menu under **Output folder location** to select the intended output folder on the configured External Storage location. 9. Input the **Reanalysis Reason**. 10. Select **Requeue Analysis**. Once the Secondary Analysis is complete, Universal Copy Service will automatically copy the analysis data to the selected output folder location. \ \ \
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