When are the run folders created after initiating a sequencing run on the NovaSeq X/X Plus?

Control computer Run folder (/usr/local/illumina/runs/****)

The Run folder on the control computer is generated at the start of the run shortly after the pre-run checks complete.

Compute engine Run folder (/usr/local/illumina/mnt/runs/)

The Run folder on the compute engine (CE) is not generated until after onboard cluster generation (OBCG) completes, which is approximately 4 hours after the run starts.

Network output Run folder

The Universal Copy Service (UCS) software will create the run folder in the network output location and start the data copy after onboard cluster generation (OBCG) completes. There is an approximate 4 hour delay from when the run starts to when data copy begins.

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