Run folder sizes and Disk Space on the MiSeq
All run folder sizes listed below should be used only as an approximation.
- Folder size will vary depending on the raw cluster density and the number of clusters passing filter for the run.
- The examples below are based on dual-indexing runs with optimal clustering and clusters passing filter greater than 80%. The following also include FASTQ files generated on the instrument by MiSeq Reporter or Local Run Manager.
MiSeq v2 Chemistry |
Kit Type |
v2 50 cycle kits |
v2 300 cycle kits |
Micro |
Nano |
v2 500 cycle kits |
Nano |
MiSeq v2 Chemistry
| | | |
Kit Type | Read Length | Reagent Kit
Configuration | Output Folder Size in GB |
v2 50
cycle kits | 2 x 25 | Standard | 14 - 16 |
v2 300
cycle kits | 2 x 150 | Standard | 16 - 18 |
Micro | 3 - 5 | | |
Nano | 0.8 - 1.2 | | |
v2 500
cycle kits | 2 x 250 | Standard | 22 - 24 |
Nano | 1.6 - 1.8 | | |
Notes:
- The MiSeq hard drive is 700 GB total.
- 141 GB are partitioned to C:\ for Operating System Files and Illumina Software Installation.
- The remaining 559 GB are allocated for D:, where run data is stored, the following way:
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