How to troubleshoot errors during the pre run Wet Checks on the NovaSeq X/X Plus

The Pre-Run Checks are a series of tests that help ensure the instrument is ready to perform a sequencing run. They consist of Software, Dry, and Wet Check stages. The Wet Checks will pierce the buffer cartridge and flow buffer through the flow cell to confirm that the fluidics circuit is performing as expected.

If the instrument is unable to verify the fluidics circuit is working as expected, the Control Software will present a notification indicating the Wet Checks were unsuccessful.

If this issue presents during the Pre-Run Checks, collect the following information and files from the instrument:

1. Remove the flow cell and visually confirm if flow cell lanes appear wet or dry

2. Inspect the flow cell gasket and the instrument flow cell holder/manifold for any liquid or precipitate

3. Collect the run setup logs from the following directory based on the instrument side that presented the error:

   1. /usr/local/illumina/runs/setup-A or ../setup-B

Once this information is collected, contact Illumina Tech Support for further assistance.

For any feedback or questions regarding this article (Illumina Knowledge Article #7951), contact Illumina Technical Support [e-mail].