# Troubleshooting

Instrumentation > NextSeq 500/550 > Troubleshooting

* [Best practices for NextSeq 500/550 pre run check failures requiring a power cycle](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000005855)
* [How to change a NextSeq 500/550 nickname](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000005911)
* [How to fix inaccurate or inverted touch screen on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007990)
* [How to perform a NextSeq 500/550 system check](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001883)
* [NextSeq 500/550 Control Software v4.0.1 "Invalid Role" error with Local Run Manager v2.4.0](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002190)
* [Optimal cluster density guidelines for the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002067)
* [Troubleshooting "Local Run Manager Error Row was updated or deleted" error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007036)
* [Troubleshooting 'Lane Pump not initialized' fluidics related error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002103)
* [Troubleshooting 'Not Found' error message on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001834)
* [Troubleshooting Access to the path 'C:\NextSeqCalibration.cfg' is denied error on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000003209)
* [Troubleshooting Cannot Update Metrics errors on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002137)
* [Troubleshooting Custom Sequencing Primers selected by mistake in manual mode on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002843)
* [Troubleshooting Cycle 1 Error, Camera Disabled, Failed to Detect Clusters on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001681)
* [Troubleshooting Local Run Manager originally caused by an internal server error on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007806)
* [Troubleshooting NVRAM or Socket Errors on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001391)
* [Troubleshooting NextSeq 500/550 (NCS 2.2 or earlier) not showing Planned Run from BaseSpace PrepTab](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007437)
* [Troubleshooting NextSeq 500/550 run stopping with "Local Run Manager Error: Bad Request" error](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002771)
* [Troubleshooting Object Reference error on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007435)
* [Troubleshooting Possible Stale Image Data Detected on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001388)
* [Troubleshooting RFID errors on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001914)
* [Troubleshooting RheodyneValve error on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001389)
* [Troubleshooting ThermoCouple\_Side TemperatureProbe not operating as expected on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007810)
* [Troubleshooting Unable to Unload Reagents on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002726)
* [Troubleshooting Universal Copy Server error on the NextSeq 500/550 (NCS 4.0+)](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002770)
* [Troubleshooting Wash State Unknown in NextSeq 500/550 Control Software (NCS) v4.0.0](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002114)
* [Troubleshooting a CavroSyringePump Error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001380)
* [Troubleshooting a Failed Camera Board on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001683)
* [Troubleshooting a Failed to Get Temperature error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001382)
* [Troubleshooting a Flow Cell Door error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000003265)
* [Troubleshooting a Flow Cell Registration Failed (Skew) warning on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002053)
* [Troubleshooting a NextSeq 500/550 An existing connection was forcibly closed by a remote host error](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002361)
* [Troubleshooting a NextSeq 500/550 Cavro Syringe Pump error](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007432)
* [Troubleshooting a RAID error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001688)
* [Troubleshooting a Remote Server Returned An Error on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001812)
* [Troubleshooting a Software Shutdown Issue Warning on the NextSeq 500/550 with NCS 4.0.X](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002449)
* [Troubleshooting a Specific Camera Disabled Failed to Detect Clusters error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001379)
* [Troubleshooting a buffer cartridge/tray stuck in the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001668)
* [Troubleshooting a flow cell registration stall during pre check on first run on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002112)
* [Troubleshooting an Async Firmware Operation Error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001680)
* [Troubleshooting an Internal Temperature was Out of Range error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007529)
* [Troubleshooting an empty output folder on the NextSeq 500/550 NCS 4.0](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000005028)
* [Troubleshooting an error related to InstrumentMaintenanceState.xml on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000003516)
* [Troubleshooting an imaging system failed pre run check error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001385)
* [Troubleshooting an initialization failure due to Proxy warning on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001684)
* [Troubleshooting blank BaseSpace login screen during run setup for Windows 10 instruments (NextSeq 500/550, MiSeq, MiniSeq and Novaseq 6000)](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000003666)
* [Troubleshooting flow cell registration failed to find fiducial on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001383)
* [Troubleshooting fluidics automation error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001384)
* [Troubleshooting issues unloading reagents on a NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000007434)
* [Troubleshooting mid run Camera Disabled error on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001378)
* [Troubleshooting no output folder created when set in the Local drive on NextSeq 500/550 (NCS 4.0)](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001685)
* [Troubleshooting pre run checks with Temperature sensor check failed: Board 0/1 on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000006373)
* [Troubleshooting presence of snr.csv Files in Temp folder on NextSeq 500/550 (NCS 4.0.2)](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000003878)
* [Troubleshooting spent reagent cartridge is full on the NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001392)
* [Troubleshooting spent reagent cartridge not detected on NextSeq 500/550](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000001689)