Troubleshooting

Instrumentation > NextSeq 500/550 > Troubleshooting

• Best practices for NextSeq 500/550 pre run check failures requiring a power cycle

• How to change a NextSeq 500/550 nickname

• How to fix inaccurate or inverted touch screen on the NextSeq 500/550

• How to perform a NextSeq 500/550 system check

• NextSeq 500/550 Control Software v4.0.1 "Invalid Role" error with Local Run Manager v2.4.0

• Optimal cluster density guidelines for the NextSeq 500/550

• Troubleshooting "Local Run Manager Error Row was updated or deleted" error on the NextSeq 500/550

• Troubleshooting 'Lane Pump not initialized' fluidics related error on the NextSeq 500/550

• Troubleshooting 'Not Found' error message on NextSeq 500/550

• Troubleshooting Access to the path 'C:\NextSeqCalibration.cfg' is denied error on NextSeq 500/550

• Troubleshooting Cannot Update Metrics errors on the NextSeq 500/550

• Troubleshooting Custom Sequencing Primers selected by mistake in manual mode on the NextSeq 500/550

• Troubleshooting Cycle 1 Error, Camera Disabled, Failed to Detect Clusters on the NextSeq 500/550

• Troubleshooting Local Run Manager originally caused by an internal server error on NextSeq 500/550

• Troubleshooting NVRAM or Socket Errors on NextSeq 500/550

• Troubleshooting NextSeq 500/550 (NCS 2.2 or earlier) not showing Planned Run from BaseSpace PrepTab

• Troubleshooting NextSeq 500/550 run stopping with "Local Run Manager Error: Bad Request" error

• Troubleshooting Object Reference error on NextSeq 500/550

• Troubleshooting Possible Stale Image Data Detected on the NextSeq 500/550

• Troubleshooting RFID errors on NextSeq 500/550

• Troubleshooting RheodyneValve error on NextSeq 500/550

• Troubleshooting ThermoCouple_Side TemperatureProbe not operating as expected on the NextSeq 500/550

• Troubleshooting Unable to Unload Reagents on the NextSeq 500/550

• Troubleshooting Universal Copy Server error on the NextSeq 500/550 (NCS 4.0+)

• Troubleshooting Wash State Unknown in NextSeq 500/550 Control Software (NCS) v4.0.0

• Troubleshooting a CavroSyringePump Error on the NextSeq 500/550

• Troubleshooting a Failed Camera Board on the NextSeq 500/550

• Troubleshooting a Failed to Get Temperature error on the NextSeq 500/550

• Troubleshooting a Flow Cell Door error on the NextSeq 500/550

• Troubleshooting a Flow Cell Registration Failed (Skew) warning on the NextSeq 500/550

• Troubleshooting a NextSeq 500/550 An existing connection was forcibly closed by a remote host error

• Troubleshooting a NextSeq 500/550 Cavro Syringe Pump error

• Troubleshooting a RAID error on the NextSeq 500/550

• Troubleshooting a Remote Server Returned An Error on NextSeq 500/550

• Troubleshooting a Software Shutdown Issue Warning on the NextSeq 500/550 with NCS 4.0.X

• Troubleshooting a Specific Camera Disabled Failed to Detect Clusters error on the NextSeq 500/550

• Troubleshooting a buffer cartridge/tray stuck in the NextSeq 500/550

• Troubleshooting a flow cell registration stall during pre check on first run on NextSeq 500/550

• Troubleshooting an Async Firmware Operation Error on the NextSeq 500/550

• Troubleshooting an Internal Temperature was Out of Range error on the NextSeq 500/550

• Troubleshooting an empty output folder on the NextSeq 500/550 NCS 4.0

• Troubleshooting an error related to InstrumentMaintenanceState.xml on the NextSeq 500/550

• Troubleshooting an imaging system failed pre run check error on the NextSeq 500/550

• Troubleshooting an initialization failure due to Proxy warning on the NextSeq 500/550

• Troubleshooting blank BaseSpace login screen during run setup for Windows 10 instruments (NextSeq 500/550, MiSeq, MiniSeq and Novaseq 6000)

• Troubleshooting flow cell registration failed to find fiducial on the NextSeq 500/550

• Troubleshooting fluidics automation error on the NextSeq 500/550

• Troubleshooting issues unloading reagents on a NextSeq 500/550

• Troubleshooting mid run Camera Disabled error on the NextSeq 500/550

• Troubleshooting no output folder created when set in the Local drive on NextSeq 500/550 (NCS 4.0)

• Troubleshooting pre run checks with Temperature sensor check failed: Board 0/1 on NextSeq 500/550

• Troubleshooting presence of snr.csv Files in Temp folder on NextSeq 500/550 (NCS 4.0.2)

• Troubleshooting spent reagent cartridge is full on the NextSeq 500/550

• Troubleshooting spent reagent cartridge not detected on NextSeq 500/550