# Troubleshooting a Specific Camera Disabled Failed to Detect Clusters error on the NextSeq 500/550

This notification indicates that the NextSeq 500/550 is unable to detect clusters at cycle 1 for a specific section, lane and/or surface(s) as opposed to all cameras/lanes/surfaces. The following article is for runs that have not stopped prematurely.

This notification is typically caused by cluster density issues or communication issues and, because the NextSeq 500/550 has multiple cameras, the run may progress to completion if other cameras/lanes/surfaces are performing.

**Potential error message:**\
![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-61e0051afc39b8b69b41636dfc68a5ffa68667ab%2Fimage1.jpg?alt=media\&token=ad08fa7a-ada7-4e00-94d1-3f88889589f7)

**Troubleshooting steps:**

1. Evaluate the run performance for other sections and lanes.

* Compare the cluster density of other lanes to the recommended cluster density for the NextSeq 500/550 (170-220k/mm2 for well balanced libraries).
* If the run is over/under clustered or shows poor performance, a sample or cluster density issue is suspected.

2. If run performance meets specifications\\\*, a communication issue is suspected; power cycle the instrument and perform the system checks.

* For further information, search for Knowledge Base articles **How to Perform a NextSeq 550 Power Cycle** and **How to Perform a NextSeq 550 System Check**.
* If all system checks pass, proceed with using the instrument normally.

3. If issue persistently occurs with same camera and high or low cluster density are not suspected, contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) for further guidance.

For further information, search for Knowledge Base articles:

* **Cluster density guidelines for Illumina sequencing platforms using non-patterned flow cells**.
* **Troubleshooting Mid-Run Camera Disabled Error on the NextSeq 500/550**
* **Troubleshooting Cycle 1 Error: Camera Disabled: Failed to Detect Clusters on the NextSeq 500/550**

\\\*Note: See the support page for [NextSeq 500/550 Specifications](https://www.illumina.com/systems/sequencing-platforms/nextseq/specifications.html).

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #1379), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000001379%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |