How to calculate the DRAGEN license quota

DRAGEN counts the number of bases in an input sample file in either FASTQ or BAM format. Any analysis using FASTQ or BAM as input will consume license. If starting from FASTQ files to perform alignment and variant calling in a single command, DRAGEN will only count quota once (i.e. quota is not counted for creating BAM/CRAM files then again for the variant calling step).

Cancelled or aborted analyses that did not complete will not be included in the quota calculation.

The bases used for DRAGEN analysis are counted as one of the final steps. The amount is then written to an encrypted file at the end of analysis. If the license quota is exceeded during an analysis, that analysis will still complete without interruption, but the user will not be able to perform any further analysis without purchasing additional quota.

For example, if the user has 50 Gigabases quota left of the license and starts analyzing a 90 Gigabases input sample, the analysis will still complete, but the user will not be able to perform another analysis.

If the quota is exceeded or the license has expired, the user will not be able to perform further analysis. Contact the Illumina sales to renew the license.

dragen_lic command line tool For versions lower than 4.3, this tool can be found at /opt/edico/bin/dragen_lic. For 4.3 and higher, it can be found at /usr/bin/dragen_lic.To check current genome license usage and expiration date, use the following command:dragen_lic -f genomeExample of the command output below: image.pngThe above license output example is for the Genome license. The first line shows that 1000 Gigabases have been consumed. The license installed is for 100 000 Gigabases, so 1% of the Gigabases has been used. The second line shows the license data and importantly, its expiry date. Past the expiration date, the license becomes expired and no further DRAGEN Analysis requiring the specific DRAGEN pipeline license can be performed.Following the license data is the license information that is stored on the dongle or USB key attached to the server. These lines show the status of all the accelerators that are enabled and they are specific to the different pipelines. The accelerators also have an expiry date which should be similar to the license for each pipeline and similar to the genome license example.