How to calculate the DRAGEN license quota

How to calculate the DRAGEN license quota (in gigabases Gb)? What happens if a user goes over the limit?

DRAGEN counts the number of bases in a sample file in either FASTQ or BAM format. Analyses using FASTQ or BAM input will consume licence. If starting from FASTQ Files, DRAGEN will only count quota once (eg, does not count quota for creating bam/cram files).

Cancelled/Aborted analyses (that did not complete) will not be included in the data quota calculation.

If the quota is exceeded, the license will expire, and the user will not be able to perform further analysis. Contact the Illumina sales to renew the license.

Note: The bases get counted up during the run and then are written to an encrypted file at the end of the analysis. If the license quota is exceeded during an analysis, that analysis will still complete fine, but the user will not be able to perform the next analysis.

For example, if the user has remained 50 Gb license and starts analysis with 90 Gbase input, the analysis will still be complete, but the user will not be able to perform the next analysis.

d****ragen_lic command line tool

For versions lower than 4.3, this tool can be found at /opt/edico/bin/dragen_lic. For 4.3 and higher, it can be found at /usr/bin/dragen_lic

To check current genome license usage and expiration date, use the following command:

dragen_lic -f genome

Example below:

The above license output example is for the Genome license. The first line shows that 1000 gigabases have been consumed. The license installed is for 100000 gigabases and 1% of the gigabases have been used.

The second line shows the license data and it is the expiry date that is important. The license expires either at the expiry date or when 100% of the licensed gigabases are consumed.

Following the license data is the license information that is stored on the dongle or USB key attached to the server. These lines show the status of all the accelerators that are enabled and they are specific to the different pipelines. The accelerators also have an expiry date which should be similar to the license for each pipeline and similar to the genome license example.