Index selection and FAQ for Illumina DNA PCR Free

Is Illumina DNA PCR-Free compatible with all Illumina indexes kits? Which indexes can be used with Illumina DNA PCR-Free kit?

• Illumina DNA PCR free is compatible with the 10 bp IDT for Illumina - DNA/RNA UD Indexes (formerly, the IDT for Illumina Nextera DNA Unique Dual Indexes).

Where are the sequences of adapters, sequencing primers, and indexes used in Illumina PCR-Free library prep?

• All publicly available sequences can be found in the Illumina Adapter Sequences Document.

• Sequencing primer sequences are proprietary.

How to choose index combinations for libraries if pooling a low number of libraries in a sequencing run?* See the IDT for Illumina-DNA/RNA UD Indexes section of the Index Adapters Pooling Guide for guidance.

• See Balancing sample coverage for whole-genome sequencing technical note for approximate expected index representations.

For any feedback or questions regarding this article (Illumina Knowledge Article #3471), contact Illumina Technical Support [e-mail].