Index selection and FAQ for Illumina DNA PCR Free

Is Illumina DNA PCR-Free compatible with all Illumina indexes kits? Which indexes can be used with Illumina DNA PCR-Free kit?

  • Illumina DNA PCR free is compatible with the 10 bp IDT for Illumina - DNA/RNA UD Indexes (formerly, the IDT for Illumina Nextera DNA Unique Dual Indexes).

Where are the sequences of adapters, sequencing primers, and indexes used in Illumina PCR-Free library prep?

  • All publicly available sequences can be found in the Illumina Adapter Sequences Document.

  • Sequencing primer sequences are proprietary.

How to choose index combinations for libraries if pooling a low number of libraries in a sequencing run?* See the IDT for Illumina-DNA/RNA UD Indexes section of the Index Adapters Pooling Guide for guidance.

For any feedback or questions regarding this article (Illumina Knowledge Article #3471), contact Illumina Technical Support techsupport@illumina.com.

Last updated

© 2023 Illumina, Inc. All rights reserved. All trademarks are the property of Illumina, Inc. or their respective owners. Trademark information: illumina.com/company/legal.html. Privacy policy: illumina.com/company/legal/privacy.html