Index selection and FAQ for Illumina DNA PCR-Free

Is Illumina DNA PCR-Free compatible with all Illumina indexes kits? Which indexes can be used with Illumina DNA PCR-Free kit?

• Illumina DNA PCR free is compatible with the Illumina DNA/RNA UD Index kits.

Where are the sequences of adapters, sequencing primers, and indexes used in Illumina PCR-Free library prep?

• All publicly available sequences can be found in the Illumina Adapter Sequences Document.

How to choose index combinations for libraries if pooling a low number of libraries in a sequencing run?

• See the Illumina DNA/RNA UD Indexes section of the Index Adapters Pooling Guide for guidance.

• See Balancing sample coverage for whole-genome sequencing technical note for approximate expected index representations.

• Index correction factors file

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