Can Local Run Manager be used to plan iSeq 100 runs with custom sequencing primers?

No, these runs must be set up in Manual mode within the iSeq 100 Control Software. Additionally, custom sequencing primers must be enabled within the control software by navigating to the control software menu and selecting System Settings > Customization > Enable Custom Primers.

For additional details, refer to the iSeq 100 Product Documentation Custom Primers section Or consult the knowledge base article Custom primer use workflow for iSeq 100 control software version 3.0 for a step-by-step overview of the workflow.

For any feedback or questions regarding this article (Illumina Knowledge Article #6500), contact Illumina Technical Support [e-mail].