Can IDT for Illumina DNA/RNA UD Indexes be used with the Illumina 16S Metagenomics Protocol?

Yes, the IDT for Illumina DNA/RNA Unique Dual (UD) Indexes can be used with the Illumina 16S Metagenomic Sequencing Library Protocol. These indexes are compatible with the overhang sequences added in the first PCR step of the workflow.

When using IDT for Illumina DNA/RNA UD Indexes for the 16S workflow, use 10 µl of the pre-combined index primers per sample, rather than 5 µl each of the i5 and i7 Nextera XT index primers.

Note: The IDT for Illumina DNA/RNA UD Indexes are 10 bp each; whereas, the Nextera XT indexes are 8 bp each.

For any feedback or questions regarding this article (Illumina Knowledge Article #5766), contact Illumina Technical Support [e-mail].