Troubleshooting a "Failed to Get Temperature" warning on the NextSeq 500/550

The following warning is typically a transient communication issue between the NextSeq Control Software and a temperature sensor, and does not affect run performance.

"@@@---Failed to get temperature."

Troubleshooting steps:

1. If run performance is normal, power cycle the instrument after run completion, and proceed with the next run if initialization is successful.

   1. For reference, see How to Power Cycle the NextSeq 500/550

2. If run performance is affected, power cycle the instrument and perform system check tests. If all tests pass, proceed with next sequencing run.

   1. For reference, see How to perform a NextSeq 500/550 system check

3. If the warning re-occurs or the system check shows a failure, contact Illumina Technical Support.

For any feedback or questions regarding this article (Illumina Knowledge Article #1382), contact Illumina Technical Support [e-mail].