Troubleshooting 'Failed to start First Time Setup' Errors on the MiSeq i100 Series Instruments

Background

The First Time Setup workflow assists with setting up an Administrator account, networking, and performing a System Health Check. If the Control Software is not able to proceed with starting the System Health Checks, the following error message displays:

Figure 1: Critical System Error: Failed to start First Time Setup: Analysis is ongoing. System Checks cannot be started.

This issue generally presents when the DRAGEN Manager service is attempting to perform the DRAGEN license check and is not getting a response, typically due to network security restrictions. If this issue presents, see the following instructions.

Troubleshooting Actions

1. Allow the instrument to remain idle for minimum of 1 hour on the error screen.

2. Select Restart application to restart the Control Software. Do not power cycle the instrument.

3. After initialization, the instrument will restart First Time Setup. Navigate through the screens, confirming that the information was correctly saved from the first attempt.

4. Once the First Time Setup is complete, proceed to starting the System Health Checks.

If the issue persists, please contact Illumina Tech Support for further assistance.

For any feedback or questions regarding this article (Illumina Knowledge Article #9239), contact Illumina Technical Support [e-mail].