How to degas the Te flow

The proper technique for degassing the Te-flow is as follows.

• Set water circulator to 44°C in preparation for XStain.

• When the temperature reaches ~ 30°C, separate the heat exchanger (black piece) from the reagent pan (white base) (A).

• Lift the heat exchanger upright and away from you (tubing at the bottom) (B) and Turn 90° counterclockwise (C).

• Turn back horizontal (A). Repeat (B) > (C) > (A) 3 times for a total of 4 rotations or until air/bubbles are no longer audible.

• Using Kimwipes dampened with lab-grade water, clean all surfaces between the heat exchanger and reagent pan as indicated by the blue arrows shown in D and E to remove reagent build-up. Discard Kimwipes with formamide waste.

• Place Te-flow back on the reagent pan and ensure it is flush using the two guide pins in the reagent pan.

  

This information is also available in video form. Search for Knowledge Base article How to degas the Te-flow Video.

For further information, search for Knowledge Base Article Infinium Array: Best Practices Support Webinar Video.

For any feedback or questions regarding this article (Illumina Knowledge Article #1479), contact Illumina Technical Support [e-mail].