# FAQ

Instrumentation > iSeq 100 > FAQ

* [Are custom primers loaded as a pool or individually on the iSeq 100?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006504)
* [Are custom primers recoverable after loading into the iSeq 100 reagent cartridge?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006505)
* [Are washes required on the iSeq 100?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006673)
* [Can Local Run Manager be used to plan iSeq 100 runs with custom sequencing primers?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006500)
* [Can PhiX be sequenced on the iSeq 100 when using custom primers?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006502)
* [Can custom primers be spiked into the Illumina primers within the iSeq 100 reagent cartridge?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006501)
* [Can custom sequencing primers be used on the iSeq 100?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006499)
* [Can iSeq 100 runs with custom primers be analyzed in Local Run Manager or BaseSpace Sequence Hub?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006507)
* [Does Illumina offer Preventative Maintenance (PM) for iSeq 100 system?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000005123)
* [Does the iSeq 100 have any special moving considerations?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000002316)
* [How many runs can be stored on the iSeq 100?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000003060)
* [How much liquid waste is inside an iSeq 100 cartridge?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006977)
* [How to determine whether custom primers were selected on the iSeq 100 once the run is completed?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006506)
* [How to dilute custom primers for use on the iSeq 100?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006503)
* [How to dispose of iSeq 100 reagents?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000005122)
* [How to minimize the iSeq 100 Control Software?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006805)
* [How to turn audio on or off on the iSeq 100?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000003271)
* [Lifespan and maximum usage of the iSeq 100 System test kit](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000007573)
* [What are the available run setup modes for iSeq 100?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000005570)
* [What does the iSeq 100 status bar color mean?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006208)
* [When do %>Q30 and %Pass Filter metrics become available for iSeq 100 runs?](https://knowledge.illumina.com/instrumentation/iseq-100/instrumentation-iseq-100-faq-list/000006806)


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