FAQ

Instrumentation > iSeq 100 > FAQ

• Are custom primers loaded as a pool or individually on the iSeq 100?

• Are custom primers recoverable after loading into the iSeq 100 reagent cartridge?

• Are washes required on the iSeq 100?

• Can Local Run Manager be used to plan iSeq 100 runs with custom sequencing primers?

• Can PhiX be sequenced on the iSeq 100 when using custom primers?

• Can custom primers be spiked into the Illumina primers within the iSeq 100 reagent cartridge?

• Can custom sequencing primers be used on the iSeq 100?

• Can iSeq 100 runs with custom primers be analyzed in Local Run Manager or BaseSpace Sequence Hub?

• Does Illumina offer Preventative Maintenance (PM) for iSeq 100 system?

• Does the iSeq 100 have any special moving considerations?

• How many runs can be stored on the iSeq 100?

• How much liquid waste is inside an iSeq 100 cartridge?

• How to determine whether custom primers were selected on the iSeq 100 once the run is completed?

• How to dilute custom primers for use on the iSeq 100?

• How to dispose of iSeq 100 reagents?

• How to minimize the iSeq 100 Control Software?

• How to turn audio on or off on the iSeq 100?

• Lifespan and maximum usage of the iSeq 100 System test kit

• What are the available run setup modes for iSeq 100?

• What does the iSeq 100 status bar color mean?

• When do %>Q30 and %Pass Filter metrics become available for iSeq 100 runs?