Specifications for the MiSeq

* Total time includes cluster generation, sequencing, and base calling on a MiSeq System enabled with dual-surface scanning.
Note: Output is measured in Gb (Gigabases) or Mb (Megabases).
Reads Passing Filter
Install specifications based on Illumina PhiX control library at supported cluster densities (1000-1200 k/mm2 raw cluster density for v2 chemistry and 1200-1400 k/mm2 raw cluster density for v3 chemistry). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.

Quality Scores
A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases Q30 is averaged across the entire run.

Imaging
The number of tiles and surfaces for each kit type.
Kit Type | Number of Tiles | Surfaces imaged |
v2 Standard | 14 tiles | Dual surface |
v2 Micro | 4 tiles | Dual surface |
v2 Nano | 2 tiles | Single surface |
v3 Standard | 19 tiles | Dual surface |
v3 VeriSeq-PGS | 19 tiles | Dual surface |
This information is also available on the Illumina MiSeq Specifications page, and a PDF version, here: MiSeq System Specifications Sheet.
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