Specifications for the MiSeq

* Total time includes cluster generation, sequencing, and base calling on a MiSeq System enabled with dual-surface scanning. Note: Output is measured in Gb (Gigabases) or Mb (Megabases).
Reads Passing Filter Install specifications based on Illumina PhiX control library at supported cluster densities (1000-1200 k/mm2 raw cluster density for v2 chemistry and 1200-1400 k/mm2 raw cluster density for v3 chemistry). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.
Quality Scores A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases Q30 is averaged across the entire run.
Imaging The number of tiles and surfaces for each kit type.
Kit Type
Number of Tiles
Surfaces imaged
v2 Standard
14 tiles
Dual surface
v2 Micro
4 tiles
Dual surface
v2 Nano
2 tiles
Single surface
v3 Standard
19 tiles
Dual surface
v3 VeriSeq-PGS
19 tiles
Dual surface
This information is also available on the Illumina MiSeq Specifications page, and a PDF version, here: MiSeq System Specifications Sheet.
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