Specifications for the MiSeq

\* Total time includes cluster generation, sequencing, and base calling on a MiSeq System enabled with dual-surface scanning. Note: Output is measured in Gb (Gigabases) or Mb (Megabases).

Reads Passing Filter Install specifications based on Illumina PhiX control library at supported cluster densities (1000-1200 k/mm2 raw cluster density for v2 chemistry and 1200-1400 k/mm2 raw cluster density for v3 chemistry). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.

Quality Scores A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases ≥ Q30 is averaged across the entire run.

Imaging The number of tiles and surfaces for each kit type.

Kit Type	Number of Tiles	Surfaces imaged
v2 Standard	14 tiles	Dual surface
v2 Micro	4 tiles	Dual surface
v2 Nano	2 tiles	Single surface
v3 Standard	19 tiles	Dual surface
v3 VeriSeq-PGS	19 tiles	Dual surface

This information is also available on the Illumina MiSeq Specifications page, and a PDF version, here MiSeq System Specifications Sheet.

For any feedback or questions regarding this article (Illumina Knowledge Article #1670), contact Illumina Technical Support [email protected].