Serial number locations on Illumina sequencers

For all Illumina Sequencers, the Instrument Serial Number is embedded into the Run ID. The Run folders in the Temp and Output folders are titled with the Run ID by default in the following format: YYMMDD_<InstrumentSerialNumber><Run Number><FlowCellBarcode>

Specific Serial Number Locations for Each Instrument

iSeq 100:

• The Temp Run folderis located at D:\Illumina\iSeq Runs[Runfolder]

• The iSeq 100 Serial Number can be found in the Control Software in the About Menu or the upper left-hand corner of the Control Software.

  
• The iSeq 100 Serial Number can be found on a sticker under the bottom front door, where the leak pad is located.

  

MiniSeq:

• Temp Run folder is located at D:\Illumina\MiniSeq Sequencing Temp[Runfolder]

• The MiniSeq Serial Number can be found in the MiniSeq Control Software in the About Menu or the upper left-hand corner of the control software.

  • From Control Software home screen, Navigate as follows: Manage Instrument > About > Instrument ID.

  

MiSeq:

• Temp Run folder is located at D:\Illumina\MiSeqTemp[Run Folder]

• In MiSeq Control Software v4.0 and later:

  • The MiSeq Serial Number is located in the upper left-hand corner of the Control Software.

  
• The MiSeq Serial Number may also be located on the back of the instrument.

  

NextSeq 500/550:

• Temp Run folder is located at D:\Illumina\ NextSeq Control Software Temp[Runfolder]

• In NextSeq Control Software v4.0 and later, the Instrument Serial Number may be found in the About Menu or the upper left-hand corner of the Control Software.

  • From the Control Software home screen, Navigate as follows: Manage Instrument > About > Instrument ID.

  

  
• The NextSeq 500/550 serial number may also be located on the back of the instrument near the bottom.

  

NextSeq 1000/2000:

• The Temp Run folderis located at /usr/local/Illumina/runs/[Runfolder]

• In the NextSeq Control Software, the serial number is located at the bottom of the home screen. Users may also navigate as follows: Hamburger Menu (Upper Left-Hand Corner) > About > Instrument Serial Number.

  

  

NovaSeq 6000**:**

• The Temp Run folder(both sides) is located at C:\ProgramData\Illumina\NovaSeq\NovaSeqTemp[Runfolder]

• The NovaSeq Serial Number is located in the upper left-hand corner of the Control Software.

  
• The NovaSeq Serial number is also located on the back of the instrument next to the label.

  

NovaSeq X Series:

• The Temp folder is under the /usr/local/illumina/runs/<RunID> folder.

• In the NovaSeq X Control Software, the Serial Number is also listed at the path Instrument Icon > Settings > About.

  

MiSeq i100 Series

• The MiSeq i100 Series serial number can be found in the settings menu by selecting the menu> About > Serial Number:

  
• The serial number can also be found on the back of the instrument near the ethernet ports

  

For any feedback or questions regarding this article (Illumina Knowledge Article #6351), contact Illumina Technical Support [e-mail].