# Serial Number locations on Illumina sequencers

For all Illumina Sequencers, the Instrument Serial Number is embedded into the Run ID. The Run folders in the **Temp** and **Output** folders are titled with the Run ID by default in the following format:

**YYMMDD\_\_\_**

Specific Serial Number Locations for Each Instrument

**iSeq** **100**:

* The Temp Run folderis located at D:\Illumina\iSeq Runs\[Runfolder].
* The iSeq 100 Serial Number can be found in the control software in the About Menu or the upper left-hand corner of the control software.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-377af4ef77aa09f9ef79c5550c8cda150e933756%2Fimage1.png?alt=media)
* The iSeq 100 Serial Number can be found on a sticker under the bottom front door, where the leak pad is located, or behind the air filter.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-b18d3dd9df48bb14b72a19fd3c36c44cc688401c%2Fimage2.png?alt=media)

**MiniSeq**:

* Temp Run folder is located at D:\Illumina\MiniSeq Sequencing Temp\<run folder>
* The MiniSeq Serial Number may be found in the MiniSeq Control Software in the About Menu or the upper left-hand corner of the control software.

  * From control software home screen, Navigate as follows: Manage Instrument > About > Instrument ID.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-ceef519935d938e8d934043e193de5f5d10f6f61%2Fimage4.png?alt=media)

\*\* ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-f319e764a01022529d31521fc8ac48f980f059fc%2Fimage5.png?alt=media)\
\*\*

**MiSeq**:

* Temp Run folder is located at D:\Illumina\MiSeqTemp\[Run Folder]
* In MiSeq Control Software (MCS) v4.0 and later:

  * The MiSeq Serial Number is located in the upper left-hand corner of the control software.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-5e5e70440dae48305c7227a48dad07712baa27e4%2Fimage6.png?alt=media)
* The MiSeq Serial Number may also be located on the back of the instrument.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-e6dcc2430fe4953e053e2613a8d206aa3a118e5f%2Fimage7.png?alt=media)

**NextSeq** **500/550:**

* Temp Run folder is located at D:\Illumina\ NextSeq Control Software Temp\<Run\_Folder>
* In NextSeq Control Software (NCS) 4.0 and later, The Instrument Serial Number may be found in the About Menu or the upper left-hand corner of the control software.

  * From the control software home screen, Navigate as follows: Manage Instrument > About > Instrument ID.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-ce82e759b6436ffef536e5dc6b0863aa6d2bcb6f%2Fimage8.png?alt=media)

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-e49c59d6846c0cc6c0efcecec4c3d5307aed708a%2Fimage9.png?alt=media)
* The NextSeq 500/550 serial number may also be located on the back of the instrument near the bottom.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-bf37ca0d0a0546cc483b5baebb3dba14938f892c%2Fimage10.png?alt=media)

**NextSeq** **1000/2000:**

* The Temp Run folderis located at /usr/local/Illumina/runs/\[runfolder].
* In the NextSeq Control Software, the serial number is located at the bottom of the home screen. Users may also navigate as follows: Hamburger Menu (Upper Left-Hand Corner) > About > Instrument Serial Number.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-ad0b253ac2a260b8103695554c8df15928b5946b%2Fimage11.png?alt=media)

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-c2ed23ac09bdcc7a2d3b1cf47b0979405dfcb97b%2Fimage12.png?alt=media)

**NovaSeq** **6000**\*\*:\*\*

* The Temp Run folder(both sides) is located at C:\ProgramData\Illumina\NovaSeq\NovaSeqTemp\[run folder]
* The NovaSeq Serial Number is located in the upper left-hand corner of the control software.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-3d794016d7488f096923a32d53b53607fad2276c%2Fimage14.png?alt=media)
* The NovaSeq Serial number is also located on the back of the instrument next to the label.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-0c4be30292a5085653cb2a0d27f6ef224bdbe785%2Fimage15.png?alt=media)

**NovaSeq** **X** **Series:**

* The Temp folder is under the /usr/local/illumina/runs/ folder.
* In the NovaSeq X Control Software, the Serial Number is also listed at the path Instrument Icon > Settings > About.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-18dc78d631e6dd30b9ace30f1c25917a7959113f%2Fimage16.png?alt=media)

  **MiSeq i100 Series**\* The MiSeq i100 Series serial number can be found in the settings menu by selecting the menu> About > Serial Number:

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-43cd606f6de9500e588d2e1103d8d6d3f87bf7b6%2Fimage19.png?alt=media)
* The serial number can also be found on the back of the instrument near the ethernet ports\
  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-53d1e4156651b96f21c7c64b11cee1a25dac6a9e%2Fimage20.png?alt=media)

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                        |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #6351), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000006351%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |