Species compatibility table for Ribo Zero Human/Mouse/Rat and Ribo Zero Gold
TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat and Ribo-Zero Gold have been laboratory tested with human, mouse, and rat samples, but may be compatible to a variety of eukaryotic species.
The following table lists organisms that have been laboratory tested and are fully supported by Illumina, tested in silico, or reported to work by non-Illumina labs. Illumina cannot guarantee rRNA removal based on in silico or non-Illumina lab reported results.
This table can also be found on the Stranded Total RNA Species Compatibility Tables webpage.
Organism | Laboratory Tested | In silico tested | Customer Reported | Comments |
Bee (Apis mellifera) | yes | Use the highest quality RNA possible. | ||
Chicken (Gallus gallus) | yes | Removes 5S, 18S and 28S rRNAs. Mitochondrial rRNAs not tested. | ||
Ciona intestinalis | yes | Removes 18S rRNA. Other rRNAs not tested. | ||
Colletotrichum higginsianum | yes | Will remove 28S and 5.8S rRNA. Will not remove 5S rRNA. 18S rRNA not evaluated. | ||
Copepod species | yes | Removes 28S and 18S rRNAs. Other rRNAs were not evaluated. | ||
Cow | yes | |||
Dictyostelium discoideum | yes | Removes 28S and 18S rRNA from high quality RNA. Will not remove small rRNAs. | ||
Dog | yes | |||
Ferret (Mustela putorius) | yes | |||
Finch (Carpodacus mexicanus) | yes | Removes 5S and 18S rRNA. Will not remove 18S rRNA. Mitochondrial rRNAs not tested. | ||
Flour beetle (Tribolium castaneum) | yes | |||
Frog (Xenopus) | yes | |||
Fruit fly (Drosophila) | yes | Will not remove 5S rRNA or 'right' fragment of 28S rRNA. | ||
Fungus (Aspergillus niger) | yes | 300 nt region of 18S rRNA will not be removed from a degraded sample. Use highest quality RNA possible. | ||
Fungus (Basidiomycete) | yes | Use highest quality RNA possible. | ||
Fungus (Candida albicans) | yes | |||
Fungus (Cryptococcus neoformans) | yes | Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible. | ||
Fungus (Yarrowia lipolytica) | yes | Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible. | ||
Horse (Equine) | yes | |||
Human | yes | yes | All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples. | |
Insect (Rhodnius prolixus) | yes | Use highest quality RNA possible. Mitochondrial rRNA not removed. | ||
Lamprey | yes | Use highest quality RNA possible. | ||
Marine sponge (Crambe crambe) | yes | Use highest quality RNA possible. | ||
Monkey (Macaque; Cynomolgus macaques) | yes | |||
Monkey (African green; Chlorocebus sabaeus) | yes | |||
Monkey (Rhesus; Macaca mulatta) | yes | |||
Mosquitoes (Anopheles gambiae) | yes | Removes 18S rRNA. Will not remove 5S, 5.8S, and 12S, 16S mitochondrial rRNA. 28S rRNA not tested. | ||
Mouse | yes | yes | All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples | |
Nematode (C. elegans) | yes | Use highest quality RNA possible. | ||
Newt (Notophthalmus viridescens) | yes | May not remove 5.8S or 5S rRNAs. Use highest quality RNA possible. | ||
Plasmodium falciparum | yes | Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible. | ||
Poeciliid fishes | yes | Removes 28S and 18S rRNA. May not remove all 5S rRNA. | ||
Rat | yes | yes | All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples | |
Salmon (Salmo salar) | yes | Removes 18S, 5.8S, and 5S rRNAs. Will not remove 28S rRNA. Mitochondrial rRNAs were not evaluated | ||
Sea anemone (Nematostella vectensis) | yes | Use highest quality RNA possible. | ||
Swine | yes | Removes 28S, 18S, 5.8S and 5S rRNAs. | ||
Toxoplasma gondii | yes | Use high quality RNA. | ||
Trypanosoma brucei | yes | Will remove 28S and 18S rRNA. Other rRNA were not evaluated | ||
Welsh Onion (Allium fistulosum) | yes | Removes 18S rRNA. Other rRNAs not tested. | ||
Zebrafish (Danio rerio) | yes |
For any feedback or questions regarding this article (Illumina Knowledge Article #2095), contact Illumina Technical Support techsupport@illumina.com. |
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