Species compatibility table for Ribo Zero Human/Mouse/Rat and Ribo Zero Gold

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat and Ribo-Zero Gold have been laboratory tested with human, mouse, and rat samples, but may be compatible to a variety of eukaryotic species.

The following table lists organisms that have been laboratory tested and are fully supported by Illumina, tested in silico, or reported to work by non-Illumina labs. Illumina cannot guarantee rRNA removal based on in silico or non-Illumina lab reported results.

This table can also be found on the Stranded Total RNA Species Compatibility Tables webpage.

Organism	Laboratory Tested	In silico tested	Customer Reported	Comments
Bee (Apis mellifera)		yes		Use the highest quality RNA possible.
Chicken (Gallus gallus)		yes		Removes 5S, 18S and 28S rRNAs. Mitochondrial rRNAs not tested.
Ciona intestinalis		yes		Removes 18S rRNA. Other rRNAs not tested.
Colletotrichum higginsianum		yes		Will remove 28S and 5.8S rRNA. Will not remove 5S rRNA. 18S rRNA not evaluated.
Copepod species		yes		Removes 28S and 18S rRNAs. Other rRNAs were not evaluated.
Cow		yes
Dictyostelium discoideum		yes		Removes 28S and 18S rRNA from high quality RNA. Will not remove small rRNAs.
Dog		yes
Ferret (Mustela putorius)		yes
Finch (Carpodacus mexicanus)		yes		Removes 5S and 18S rRNA. Will not remove 18S rRNA. Mitochondrial rRNAs not tested.
Flour beetle (Tribolium castaneum)			yes
Frog (Xenopus)		yes
Fruit fly (Drosophila)			yes	Will not remove 5S rRNA or 'right' fragment of 28S rRNA.
Fungus (Aspergillus niger)		yes		300 nt region of 18S rRNA will not be removed from a degraded sample. Use highest quality RNA possible.
Fungus (Basidiomycete)		yes		Use highest quality RNA possible.
Fungus (Candida albicans)			yes
Fungus (Cryptococcus neoformans)		yes		Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible.
Fungus (Yarrowia lipolytica)		yes		Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible.
Horse (Equine)			yes
Human	yes		yes	All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples.
Insect (Rhodnius prolixus)		yes		Use highest quality RNA possible. Mitochondrial rRNA not removed.
Lamprey		yes		Use highest quality RNA possible.
Marine sponge (Crambe crambe)		yes		Use highest quality RNA possible.
Monkey (Macaque; Cynomolgus macaques)		yes
Monkey (African green; Chlorocebus sabaeus)		yes
Monkey (Rhesus; Macaca mulatta)		yes
Mosquitoes (Anopheles gambiae)		yes		Removes 18S rRNA. Will not remove 5S, 5.8S, and 12S, 16S mitochondrial rRNA. 28S rRNA not tested.
Mouse	yes		yes	All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples
Nematode (C. elegans)		yes		Use highest quality RNA possible.
Newt (Notophthalmus viridescens)		yes		May not remove 5.8S or 5S rRNAs. Use highest quality RNA possible.
Plasmodium falciparum		yes		Will not remove 5.8S or 5S rRNAs. Use highest quality RNA possible.
Poeciliid fishes			yes	Removes 28S and 18S rRNA. May not remove all 5S rRNA.
Rat	yes		yes	All tissues except blood; use TruSeq Stranded Total RNA-with Ribo-Zero Globin for blood samples
Salmon (Salmo salar)		yes		Removes 18S, 5.8S, and 5S rRNAs. Will not remove 28S rRNA. Mitochondrial rRNAs were not evaluated
Sea anemone (Nematostella vectensis)		yes		Use highest quality RNA possible.
Swine			yes	Removes 28S, 18S, 5.8S and 5S rRNAs.
Toxoplasma gondii		yes		Use high quality RNA.
Trypanosoma brucei		yes		Will remove 28S and 18S rRNA. Other rRNA were not evaluated
Welsh Onion (Allium fistulosum)		yes		Removes 18S rRNA. Other rRNAs not tested.
Zebrafish (Danio rerio)		yes

For any feedback or questions regarding this article (Illumina Knowledge Article #2095), contact Illumina Technical Support [e-mail].