For the complete documentation index, see llms.txt. This page is also available as Markdown.
How to troubleshoot MaxWetS2SpotIntensity Errors during Wet Pre Run Checks on the NovaSeq X Series
Background
The Wet Pre-Run Checks (PRCs) flow a small amount of buffer through the flow cell to verify the instrument fluidics are performing as expected. Part of this routine verifies the instrument can locate the flow cell surfaces. If the intensities from this check are above the saturation limit, the Wet PRCs fail with an error referencing the MaxWetS2SpotIntensity.
Error message:
"One or more errors occurred. (MaxWetS2SpotIntensity with value 61071 was greater than 60000)."
Troubleshooting Actions
Collect the run setup logs from the following directory based on the instrument side that presented the error:
/usr/local/illumina/runs/setup-A or ../setup-B
Once this information is collected, contact Illumina Tech Support for further assistance.
For any feedback or questions regarding this article (Illumina Knowledge Article #8269), contact Illumina Technical Support techsupport@illumina.com.
