RTA4 Q Score Bin Ranges on the NovaSeq X/X Plus
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Background
The NovaSeq X/X Plus utilizes Real Time Analysis v4 (RTA4) for base calling and quality scoring. There are three Q score bins used on the NovaSeq X/X Plus, and these Q score calls are reported as an average for the range of called scores. Please see below for the relevant Q Score Bins and Ranges depending on Control Software version.
Control Software v1.3
Bin
Q Score Range
2
NoCall, 0-2
9
3-17
24
18-29
40
30+
Control Software v1.2 and v1.2.2
Bin
Q Score Range
2
NoCall, 0-2
12
3-17
24
18-29
40
30+
For more information on changes to Q Score bins with Control Software v1.2, please see the following Illumina article:
For any feedback or questions regarding this article (Illumina Knowledge Article #8320), contact Illumina Technical Support .