RTA4 Q Score Bin Ranges on the NovaSeq X/X Plus

Background

The NovaSeq X/X Plus utilizes Real Time Analysis v4 (RTA4) for base calling and quality scoring. There are three Q score bins used on the NovaSeq X/X Plus, and these Q score calls are reported as an average for the range of called scores. Please see below for the relevant Q Score Bins and Ranges depending on Control Software version.

Control Software v1.3

Bin	Q Score Range
2	NoCall, 0-2
9	3-17
24	18-29
40	30+

Control Software v1.2 and v1.2.2

Bin	Q Score Range
2	NoCall, 0-2
12	3-17
24	18-29
40	30+

For more information on changes to Q Score bins with Control Software v1.2, please see the following Illumina article:

NovaSeq X v1.2 software enables sequencing with 80% of bases >= Q40

For any feedback or questions regarding this article (Illumina Knowledge Article #8320), contact Illumina Technical Support [e-mail].