Troubleshooting NovaSeq 6000 Reagent Chiller outside acceptable range warning

The NovaSeq 6000 reagent chiller remains at room temperature when the instrument is idle. At the start of a sequencing run, the instrument will begin cooling the reagent chiller compartment (RCA) on the instrument and will target a temperature of 7-8°C and maintain that temperature for the duration of the run. Issues with maintaining the appropriate reagent chiller temperature can cause run quality issues.

Issues during the run, if present, will often present the following warning message:

21-06-08 13:28:28.818 -05 0208 WRN: The chiller returned a temperature of 23.0289993286133 at the beginning of sequencing, which is outside the acceptable range of [4, 14] -- SequenceCommand.cs, CheckChillerTemperature, 2729

Troubleshooting steps:

• Check if the ambient temperature is significantly higher than expected.

  • Maintain a lab temperature of 19°C to 25°C (22°C ± 3°C). For further information, see NovaSeq Sequencing System Site Prep Guide.

• Collect Run Cycle logs: C:\ProgramData\*\Illumina\NovaSeq\NovaSeqTemp[run folder]\Logs (zipped)

  • After collecting the logs, contact Illumina Technical Support to determine the next steps.

For any feedback or questions regarding this article (Illumina Knowledge Article #5499), contact Illumina Technical Support [e-mail].