# Can the MiSeq System generate FASTQ files?

**Can the MiSeq System generate FASTQ files?**\
Yes. FASTQ files are generated during secondary analysis by MiSeq Reporter (MiSeq Software 2.6.2 or below) or Local Run Manager (MiSeq Software 3.1 or above) for most analysis workflows.\
To generate only FASTQ files, specify the GenerateFASTQ workflow in the sample sheet or during run planning in Local Run Manager to generate FASTQ files with no subsequent on-board analysis steps.

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                 |
| :-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #2606), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002606%20-%20Software%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://knowledge.illumina.com/software/on-instrument-analysis-software/software-on-instrument-analysis-software-faq-list/000002606.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.
