Pooling recommendations for the AmpliSeq for Illumina Comprehensive Panel v3
The AmpliSeq for Illumina Comprehensive Panel v3 creates libraries from DNA and RNA. Refer to the table below for instrument specific pooling recommendations.
Instrument and sequencing chemistry
Maximum DNA only
Maximum RNA only
Maximum DNA+RNA
DNA:RNA pooling volume ratio
MiniSeq Mid Output
N/A
24
N/A
25 DNA : 1 RNA
MiniSeq High Output
3
80
3 (3 DNA + 3 RNA)
MiSeq v2
N/A
48
N/A
MiSeq v3
3
80
3 (3 DNA + 3 RNA)
NextSeq Mid Output
16
N/A
16 (16 DNA + 16 RNA)
NextSeq High Output
48
N/A
48 (48 DNA + 48 RNA)
Note: the table above is not exhaustive; AmpliSeq for Illumina Comprehensive Panel v3 can be sequenced on other instruments as well. The sequencers listed are selected based on data output per run.
Note: The number of samples pooled per run can be increased or decreased to target lower or higher coverage per sample, respectively.
See the AmpliSeq for Illumina Comprehensive Panel v3 Compatible Products page for more information.
For any feedback or questions regarding this article (Illumina Knowledge Article #1042), contact Illumina Technical Support techsupport@illumina.com.
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