# How Can I Tell If I Sequenced Through the Insert? Part 2 Video

This video shows how to use Sequencing Analysis Viewer (SAV) plots to determine if the sequencing insert is too short. It also explores how to use the adaptertrimming.txt file on the MiSeq to diagnose short inserts.

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{% embed url="<https://www.youtube.com/watch?v=f1hep--6Uow&ab_channel=Illumina>" %}

Video length: 4:46 min

This information is also available in written form. For further information, search for Knowledge Base article **How short inserts affect sequencing performance.**

**Note:** Audio may not play properly on some headphones or speakers for this video. Please use equipment appropriate for stereo sound.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #5725), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000005725%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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