How to import files into a BaseSpace Project using the web Interface

The following file types can be uploaded to projects in BaseSpace Sequence Hub.

• FASTQ files (\*.fastq.gz),

• Analysis files (Variant Call Format (VCF) and Genomic Variant Call Format (gVCF)),

• Manifests (\*.txt),

• Sample Sheets (.csv),

• etc.

Note: User must have write access (ownership) of the BaseSpace project.

To upload data via the web interface:

1. Open the BaseSpace project.

2. From the project, select File (the paper icon), then Upload, and then select Files.

3. Select type of files to upload.

Note: Uploading multiple FASTQ, VCF, or manifest files in a single session requires files of the same type.

1. If uploading a FASTQ file, do the following.

   1. Select Select Biosample, then select or create a Biosample for the FASTQ file(s).

   2. Enter a library name.

   3. Select a library prep kit.

   4. [Optional] Enter a position name.

   5. Select Finish Upload.

2. If uploading a VCF file, do the following.

   1. Select Select Biosample, then select or create a Biosample for the VCF(s).

   2. Select Finish Upload.

3. If uploading a manifest file, do the following.

   1. Select Select Biosample, then select or create a Biosample that the manifest will be associated with.

   2. Select Finish Upload.

4. If uploading other file types, do the following. (This option is only available in New mode.)

   1. [Optional] Select Select Biosample, then select or create a Biosample that the files will be associated with.

   2. Select Finish Upload.

See the Import Files Into a Project help page for additional information.

For any feedback or questions regarding this article (Illumina Knowledge Article #3651), contact Illumina Technical Support [e-mail].