For the complete documentation index, see llms.txt. This page is also available as Markdown.
Chemistry and Imaging on MiniSeq
Two-channel SBS simplifies nucleotide detection by using a combination of two fluorescent dyes and two images to determine all four base calls.
Images taken on the MiniSeq use red and green filter bands. Thymines (T) are labeled with a green fluorophore, cytosines (C) are labeled with a red fluorophore, and adenines (A) are labeled with both red and green fluorophores. Guanines (G) are permanently dark.
The MiniSeq, NextSeq 500/550 and NovaSeq 6000 Systems use red/green two-channel chemistry technology. The NextSeq 1000/2000 uses blue/green two-channel chemistry where the blue laser replaces the red laser of the other two-channel chemistry platforms.
For more information, see the Sequencing by Synthesis page here.
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