Reagent Cartridge Layout for NovaSeq 6000

All reagent cartridges contain reagent-adjacent empty wells for the post-run wash.

Cluster cartridge

• Every other row in the cartridge is a WASH row. So Well 1 on the cartridge is the wash well for the reagent in well 5 (custom primer 1), etc.

• A denaturation reagent in position #30 contains formamide, which is an organic amide and reproductive toxin.

• To facilitate safe disposal of any unused reagent after the sequencing run, this reservoir is removable.

• Do not stack cluster cartridges, as this can dislodge formamide position.

  
• Position 17: 0.24% NaOCl

• Positions 5-7: custom primer positions

  • If using, specify during run setup, use HT1 to dilute

  • Position 5: Custom read 1 primer

  • Position 6: Custom read 2 primer

  • Position 7: Custom Index 1 primer and Custom Index 2 (for v1.5 kits)

• Positions 24, 13, 23: Illumina primer positions

• Position 24: Illumina read 1 primer

• Position 13: Illumina read 2 primer

• Position 23: Illumina index 1 and index 2 primers

SBS cartridge

Buffer cartridge

• Weighs up to 15 lbs (6.8 kg).

• Wash buffers are pumped through lines to wash after each reagent pull or imaging cycle.

• Recyclable plastic (only cartridge that can be recycled).

For further information, see Knowledge Base NovaSeq 6000 flow cell types and their compatibility with NovaSeq Control Software versions article.