# Reagent Cartridge Layout for NovaSeq 6000
All reagent cartridges contain reagent-adjacent empty wells for the post-run wash.
***Cluster cartridge***
* **Every other row in the cartridge is a WASH row. So Well 1 on the cartridge is the wash well for the reagent in well 5 (custom primer 1), etc.**
* A denaturation reagent in position #30 contains formamide, which is an organic amide and reproductive toxin.
* To facilitate safe disposal of any unused reagent after the sequencing run, this reservoir is removable.
* Do **not** stack cluster cartridges, as this can dislodge formamide position.
* Position 17: 0.24% NaOCl
* Positions 5-7: custom primer positions
* If using, specify during run setup, use HT1 to dilute
* Position 5: Custom read 1 primer
* Position 6: Custom read 2 primer
* Position 7: Custom Index 1 primer and Custom Index 2 (for v1.5 kits)
* Positions 24, 13, 23: Illumina primer positions
* Position 24: Illumina read 1 primer
* Position 13: Illumina read 2 primer
* Position 23: Illumina index 1 and index 2 primers
***SBS cartridge***
***Buffer cartridge***
* Weighs up to 15 lbs (6.8 kg).
* Wash buffers are pumped through lines to wash after each reagent pull or imaging cycle.
* Recyclable plastic (only cartridge that can be recycled).\
For further information, see Knowledge Base [NovaSeq 6000 flow cell types and their compatibility with NovaSeq Control Software versions](https://knowledge.illumina.com/instrumentation/novaseq-6000/instrumentation-novaseq-6000-reference_material-list/000002418) article.
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| *For any feedback or questions regarding this article (Illumina Knowledge Article #2215), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002215%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |
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