Reagent Cartridge Layout for NovaSeq 6000

All reagent cartridges contain reagent-adjacent empty wells for the post-run wash.

Cluster cartridge

• Every other row in the cartridge is a WASH row. So Well 1 on the cartridge is the wash well for the reagent in well 5 (custom primer 1), etc.

• A denaturation reagent in position #30 contains formamide, which is an organic amide and reproductive toxin.

• To facilitate safe disposal of any unused reagent after the sequencing run, this reservoir is removable.

• Do not stack cluster cartridges, as this can dislodge formamide position.

  
• Position 17: 0.24% NaOCl

• Positions 5-7: custom primer positions

  • If using, specify during run setup, use HT1 to dilute

  • Position 5: Custom read 1 primer

  • Position 6: Custom read 2 primer

  • Position 7: Custom Index 1 primer and Custom Index 2 (for v1.5 kits)

• Positions 24, 13, 23: Illumina primer positions

• Position 24: Illumina read 1 primer

• Position 13: Illumina read 2 primer

• Position 23: Illumina index 1 and index 2 primers

SBS cartridge

Buffer cartridge

• Weighs up to 15 lbs (6.8 kg).

• Wash buffers are pumped through lines to wash after each reagent pull or imaging cycle.

• Recyclable plastic (only cartridge that can be recycled).

For further information, see Knowledge Base NovaSeq 6000 flow cell types and their compatibility with NovaSeq Control Software versions article.

For any feedback or questions regarding this article (Illumina Knowledge Article #2215), contact Illumina Technical Support [e-mail].