# Reagent Cartridge Layout for NovaSeq 6000

All reagent cartridges contain reagent-adjacent empty wells for the post-run wash.

***Cluster cartridge***

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-f9ce73e2decfe32eabb7cdbca5f2d2a68c9092d2%2Fimage1.jpg?alt=media\&token=4b3d9a19-d925-471f-b302-558b503529e6)

* **Every other row in the cartridge is a WASH row. So Well 1 on the cartridge is the wash well for the reagent in well 5 (custom primer 1), etc.**
* A denaturation reagent in position #30 contains formamide, which is an organic amide and reproductive toxin.
* To facilitate safe disposal of any unused reagent after the sequencing run, this reservoir is removable.
* Do **not** stack cluster cartridges, as this can dislodge formamide position.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-5df66471b2fed06555af44ff40c5846243d25f77%2Fimage2.png?alt=media\&token=a90a2057-19ed-4bf0-bc00-3d67575c095c)
* Position 17: 0.24% NaOCl
* Positions 5-7: custom primer positions
  * If using, specify during run setup, use HT1 to dilute
  * Position 5: Custom read 1 primer
  * Position 6: Custom read 2 primer
  * Position 7: Custom Index 1 primer and Custom Index 2 (for v1.5 kits)
* Positions 24, 13, 23: Illumina primer positions
* Position 24: Illumina read 1 primer
* Position 13: Illumina read 2 primer
* Position 23: Illumina index 1 and index 2 primers

***SBS cartridge***

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-29da76e0ebd4cf82e6a63a94004ceb98a513982d%2Fimage3.jpg?alt=media)

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-aafe2b6acb651be1ce33e9282028da61560a1708%2Fimage4.png?alt=media)

***Buffer cartridge***

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-b3fe95f8212911caf88f595e919d7f04eb1fe36a%2Fimage5.jpg?alt=media)

* Weighs up to 15 lbs (6.8 kg).
* Wash buffers are pumped through lines to wash after each reagent pull or imaging cycle.
* Recyclable plastic (only cartridge that can be recycled).\
  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-5dc3aa8eec9212d56220f6569d1ffe0016b5df34%2Fimage6.jpg?alt=media)

For further information, see Knowledge Base [NovaSeq 6000 flow cell types and their compatibility with NovaSeq Control Software versions](https://knowledge.illumina.com/instrumentation/novaseq-6000/instrumentation-novaseq-6000-reference_material-list/000002418) article.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #2215), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002215%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |