# PhiX Control v3文库是什么，它在Illumina下一代测序中起到什么作用？

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PhiX Control v3文库（通常指PhiX，货号是[FC-110-3001](http://www.illumina.com/products/phix_control_v3.html))  来源于小型，特征明确的噬菌体PhiX基因组。它是一个平均片段长度为500bp并且由平衡碱基组成（GC碱基占比约45% ，AT碱基占比约55%）的浓缩型Illumina文库 （体积10µl，浓度10nM）。\
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1\. 校准质控

PhiX Control v3文库可以单独测序以作为以下项目的校准质控：

* 串扰矩阵的生成 （Cross talk matrix generation）
* Phasing和prephasing的计算
* Illumina测序平台整体性能的检测

更详细的信息请参考如下链接：

* [PhiX loading concentrations for validation runs on Illumina sequencing platforms](https://support.illumina.com/bulletins/2016/10/phix-loading-concentrations-for-validation-runs-on-illumina-sequencing-platforms.html)
* [How to set up the sample sheet for a PhiX validation run on the MiSeq system using Illumina Experiment Manager](https://support.illumina.com/bulletins/2016/03/how-to-set-up-the-sample-sheet-for-a-phix-validation-run-on-the-miseq-system-using-illumina-experiment-manager.html)
* [How to set up a PhiX validation run on the NextSeq 500/550 in manual mode](https://support.illumina.com/bulletins/2016/03/how-to-set-up-a-phix-validation-run-on-the-nextseq-in-standalone-mode.html)
* [How to set up a PhiX validation run on the NextSeq 500/550 system using BaseSpace Hub Prep tab](https://support.illumina.com/bulletins/2016/04/how-to-set-up-a-phix-validation-run-on-the-nextseq-system-using-.html)
* [How to set up a PhiX validation run on the NextSeq 1000/2000 sequencing platform](https://support.illumina.com/bulletins/2021/03/how-to-set-up-a-phix-validation-run-on-the-nextseq-1000-2000-seq.html)
* [How to set up a PhiX validation run on the NovaSeq 6000 sequencing platform](https://support.illumina.com/bulletins/2017/08/how-to-set-up-a-phix-validation-run-on-the-novaseq-sequencing-pl.html)
* [Local Run Manager: How to set up a PhiX validation run](https://support.illumina.com/bulletins/2017/01/local-run-manager--how-to-set-up-a-phix-validation-run.html)

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2\. 监测测序质量

由于PhiX Control v3文库小且核苷酸组成平衡，因此它也可作为监测测序实验运行质量（例如簇生成效果，测序运行进展和序列比对占比）的理想内对照（通常掺入≥1％）。\
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3\. 均衡荧光信号

对于低多样性的文库，PhiX Control v3文库在每个循环提供均衡的荧光信号，以提高整体测序质量。有关详细信息，请参阅：

[什么是核苷酸多样性，它为什么十分重要？](https://knowledge.illumina.com/instrumentation/general/instrumentation-general-reference_material-list/000007132)

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