# PhiX Control v3文库是什么，它在Illumina下一代测序中起到什么作用？
\
PhiX Control v3文库（通常指PhiX，货号是[FC-110-3001](http://www.illumina.com/products/phix_control_v3.html)) 来源于小型，特征明确的噬菌体PhiX基因组。它是一个平均片段长度为500bp并且由平衡碱基组成（GC碱基占比约45% ，AT碱基占比约55%）的浓缩型Illumina文库 （体积10µl，浓度10nM）。\
1\. 校准质控
PhiX Control v3文库可以单独测序以作为以下项目的校准质控：
* 串扰矩阵的生成 （Cross talk matrix generation）
* Phasing和prephasing的计算
* Illumina测序平台整体性能的检测
更详细的信息请参考如下链接：
* [PhiX loading concentrations for validation runs on Illumina sequencing platforms](https://support.illumina.com/bulletins/2016/10/phix-loading-concentrations-for-validation-runs-on-illumina-sequencing-platforms.html)
* [How to set up the sample sheet for a PhiX validation run on the MiSeq system using Illumina Experiment Manager](https://support.illumina.com/bulletins/2016/03/how-to-set-up-the-sample-sheet-for-a-phix-validation-run-on-the-miseq-system-using-illumina-experiment-manager.html)
* [How to set up a PhiX validation run on the NextSeq 500/550 in manual mode](https://support.illumina.com/bulletins/2016/03/how-to-set-up-a-phix-validation-run-on-the-nextseq-in-standalone-mode.html)
* [How to set up a PhiX validation run on the NextSeq 500/550 system using BaseSpace Hub Prep tab](https://support.illumina.com/bulletins/2016/04/how-to-set-up-a-phix-validation-run-on-the-nextseq-system-using-.html)
* [How to set up a PhiX validation run on the NextSeq 1000/2000 sequencing platform](https://support.illumina.com/bulletins/2021/03/how-to-set-up-a-phix-validation-run-on-the-nextseq-1000-2000-seq.html)
* [How to set up a PhiX validation run on the NovaSeq 6000 sequencing platform](https://support.illumina.com/bulletins/2017/08/how-to-set-up-a-phix-validation-run-on-the-novaseq-sequencing-pl.html)
* [Local Run Manager: How to set up a PhiX validation run](https://support.illumina.com/bulletins/2017/01/local-run-manager--how-to-set-up-a-phix-validation-run.html)
2\. 监测测序质量
由于PhiX Control v3文库小且核苷酸组成平衡，因此它也可作为监测测序实验运行质量（例如簇生成效果，测序运行进展和序列比对占比）的理想内对照（通常掺入≥1％）。\
3\. 均衡荧光信号
对于低多样性的文库，PhiX Control v3文库在每个循环提供均衡的荧光信号，以提高整体测序质量。有关详细信息，请参阅：
[什么是核苷酸多样性，它为什么十分重要？](https://knowledge.illumina.com/instrumentation/general/instrumentation-general-reference_material-list/000007132)
\
\
\
| |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #7130), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000007130%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |
---
# Agent Instructions: Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.
Perform an HTTP GET request on the current page URL with the `ask` query parameter:
```
GET https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007130.md?ask=
```
The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.
Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.