Best practices for NextSeq 500/550 pre run check failures requiring a power cycle

Note: A mitigation has been implemented in NCS v4.2 which prevents the consumables from being marked as used if they do remain in the instrument during a power cycle.

For instruments operating on a Control Software version older than v4.2, the workflow still advises to remove all consumables prior to power cycling the instrument.

Some pre-run check failures will require a power cycle for resolution. In these instances, remove the consumables from the instrument prior to power cycling. To remove the consumables prior to power cycling, complete the steps listed below:

1. Navigate to the Home Screen.

2. Select Manage Instrument.

3. Select Unload Consumable.

4. Remove all Consumables.

5. Perform a Power Cycle.\*

• This is best practice for any NextSeq 500/550 instruments (all NextSeq Control Software (NCS) versions) to prevent issues in detecting consumables.

\*For further information, search for Knowledge Base article How to Perform a NextSeq 500/550 Power Cycle.

For any feedback or questions regarding this article (Illumina Knowledge Article #5855), contact Illumina Technical Support [e-mail].