Best practices for NextSeq 500/550 pre run check failures requiring a power cycle
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Note: A mitigation has been implemented in NCS v4.2 which prevents the consumables from being marked as used if they do remain in the instrument during a power cycle.
For instruments operating on a Control Software version older than v4.2, the workflow still advises to remove all consumables prior to power cycling the instrument.
Some pre-run check failures will require a power cycle for resolution. In these instances, remove the consumables from the instrument prior to power cycling. To remove the consumables prior to power cycling, complete the steps listed below:
Navigate to the Home Screen.
Select Manage Instrument.
Select Unload Consumable.
Remove all Consumables.
Perform a Power Cycle.\*
This is best practice for any NextSeq 500/550 instruments (all NextSeq Control Software (NCS) versions) to prevent issues in detecting consumables.
\*For further information, search for Knowledge Base article How to Perform a NextSeq 500/550 Power Cycle.
For any feedback or questions regarding this article (Illumina Knowledge Article #5855), contact Illumina Technical Support .