Troubleshooting 'Insufficient memory to continue the execution of the program' errors on the NovaSeq X Plus

Background

There are several imaging preparation steps that occur during the Pre-Run Checks and after clustering has taken place. If the Control Software is unable to locate one of the surfaces during these preparation steps, it can cause runs to stop due to exhaustion of available memory on the Compute Engine (CE).

If this occurs, the runs will not be able to progress and the following error message will present to the user:

"Failed executing Protocol 2Read2Index after 04:56:50.0386667 hh:mm:ss. Insufficient memory to continue the execution of the program."

Additionally, this issue is distinct from 'Insufficient Disk Space' errors that occur during Pre-Run Checks. If issue with insufficient disk space are presented by the Control Software, see the Knowledge Article Troubleshooting Insufficient Storage Space Errors during Pre-Run Checks on the NovaSeq X Series.

Troubleshooting Actions

If this errors message occurs, see the following Troubleshooting Actions and contact Illumina Tech Support.

1. Minimize the Control Software.

   1. Select the instrument icon, then select Settings, then Minimize.

2. Select the Activities menu in the top-left corner, then open the Files application.

3. Collect the following logs from the listed locations:

   1. Run Logs: /usr/local/illumina/runs//Logs/Logs.zip

   2. Control Software logs: /usr/local/illumina/logs/novaseq-x-series-control-software

4. Contact Illumina Tech Support to request a file upload link and for next steps.

For any feedback or questions regarding this article (Illumina Knowledge Article #8636), contact Illumina Technical Support [e-mail].