> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-reference_material-list/000001049.md).

# Pooling recommendations for the AmpliSeq for Illumina Focus Panel

The AmpliSeq for Illumina Focus Panel creates libraries from DNA and RNA. Refer to the table below for instrument specific pooling recommendations.

| Instrument | Max DNA only | Max RNA only | Max DNA/RNA\*   | Pooling volume ratio |
| ---------- | ------------ | ------------ | --------------- | -------------------- |
| MiniSeq MO | 24           | 48           | 16 DNA + 16 RNA | 7 DNA : 3 RNA        |
| MiniSeq HO | 72           |              | 48 DNA + 48 RNA |                      |
| MiSeq v2   | 40           | 96           | 30 DNA+ 30 RNA  |                      |
| MiSeq v3   | 72           |              | 48 DNA + 48 RNA |                      |

Additional pooling recommendations can be found in the [Product Compatibility section of AmpliSeq Focus Panel support page](https://support.illumina.com/sequencing/sequencing_kits/ampliseq-for-illumina-focus-panel/product-compatibility.html).

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                              |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #1049), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000001049%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-reference_material-list/000001049.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.