Pooling recommendations for the AmpliSeq for Illumina Focus Panel
Last updated
Last updated
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The AmpliSeq for Illumina Focus Panel creates libraries from DNA and RNA. Refer to the table below for instrument specific pooling recommendations.
Additional pooling recommendations can be found in the Product Compatibility section of AmpliSeq Focus Panel support page.
Instrument
Max DNA only
Max RNA only
Max DNA/RNA*
Pooling volume ratio
MiniSeq MO
24
48
16 DNA + 16 RNA
7 DNA : 3 RNA
MiniSeq HO
72
48 DNA + 48 RNA
MiSeq v2
40
96
30 DNA+ 30 RNA
MiSeq v3
72
48 DNA + 48 RNA
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