Pooling recommendations for the AmpliSeq for Illumina Focus Panel

The AmpliSeq for Illumina Focus Panel creates libraries from DNA and RNA. Refer to the table below for instrument specific pooling recommendations.

Instrument	Max DNA only	Max RNA only	Max DNA/RNA*	Pooling volume ratio
MiniSeq MO	24	48	16 DNA + 16 RNA	7 DNA : 3 RNA
MiniSeq HO	72		48 DNA + 48 RNA
MiSeq v2	40	96	30 DNA+ 30 RNA
MiSeq v3	72		48 DNA + 48 RNA

Additional pooling recommendations can be found in the Product Compatibility section of AmpliSeq Focus Panel support page.

For any feedback or questions regarding this article (Illumina Knowledge Article #1049), contact Illumina Technical Support [e-mail].