# TruSight Oncology 500 and TruSight Oncology 500 HT Introduction & Overview Support Webinar Video

TruSight Oncology 500 is a next-generation sequencing tumor profiling assay that analyzes 523 genes for cancer-related biomarkers with FFPE samples. The assay supports the identification of DNA and RNA variants implicated in various tumor types, including measuring key immunotherapy biomarkers such as tumor mutation burden (TMB) and microsatellite instability (MSI). This webinar focuses on introducing TruSight Oncology 500/TruSight Oncology 500 High Throughput (HT) library preparation and is targeted at new users of the workflow. The following topics are covered: how to successfully prepare and sequence TruSight Oncology 500 libraries, comparison of TruSight Oncology 500 and TruSight Oncology 500 High Throughput workflows, and how to assess key sequencing metrics.

{% embed url="<https://www.youtube.com/watch?v=rFBu_qabHIc&ab_channel=Illumina>" %}

Video length: 1:03:43 min

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #8046), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000008046%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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