Steps if insufficient wash solution was used in a manual wash on the NextSeq 500/550 or MiniSeq

If there is not enough wash solution in the wash tray during a manual wash, this can introduce air into the system which can negatively impact subsequent sequencing run performance.

To fix this, Illumina recommends performing 2-3 maintenance washes on the instrument to flush the fluidics lines and remove any air bubbles.

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