Troubleshooting MiniSeq Z stages are not ready initialization error

If a loaded flow cell is not seated correctly during a power cycle, the MiniSeq Control Software (MnCS) will fail initialization with the following error:

  • "The following Z stages are not ready after initializing: 1"

  • "1 devices failed initialization and 2 devices were never initialized: ImagingModule: Initialize FAILED, Fluidics: NOT Initialized, Instrument: NOT Initialized (Illumina.Instrument.Logic.ApplicationBase.DeviceException)"

If this error occurs, contact Illumina Technical Support at techsupport@illumina.com for assistance.

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