FAQ

Library Preparation > General > FAQ

• Are the Illumina RNA Index Anchors the same in every well of the plate?

• BLT-PF reagent handling for the Illumina DNA PCR-Free workflow questions

• BLT-PF usage FAQ for Illumina DNA PCR-Free

• Can RSB be used as a substitute for RSB + Tween when sequencing on the NextSeq 1000/2000?

• Can samples be sent to Illumina for sequencing; does Illumina offer sequencing as a service?

• Consumable FAQ for the Illumina 5-Base Whole Genome Sequencing (WGS) and Enrichment kits

• Consumables and Equipment FAQ for the TruPath Genome assay

• Control (GDMC and SFMC) FAQ for the Illumina 5-Base Whole Genome Sequencing and Enrichment kits

• Design and coverage of the Respiratory Virus Oligos Panel v2 (RVOP v2)

• Does library loading differ between MiSeq Micro and Nano flow cells?

• FAQs on the Illumina DNA Prep, (M) Tagmentation Library Preparation protocol

• Frequently Asked Questions for Illumina FFPE DNA Prep with Exome 2.5 Enrichment

• General FAQ for Illumina Rapid RNA kits

• General FAQ for the Illumina 5-Base WGS and Enrichment kits

• General workflow FAQ for the Illumina 5-Base WGS and Enrichment kits

• How many samples can be prepared with the Illumina Exome 2.5 panel?

• How many samples can be prepared with the Respiratory Pathogen ID/AMR Enrichment Panel (RPIP) Kit?

• How much PhiX to spike in for well-balanced, base diverse libraries?

• How to pool Illumina DNA Prep with Exome 2.5 libraries on the NextSeq series and NovaSeq 6000?

• How to stagger sequences or heterogeneity spacers introduce diversity for amplicon libraries?

• Hybridization Buffer (HT1) and Resuspension Buffer (RSB) alternatives and purchasing availability

• IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage

• Index and UMI information for the Illumina miRNA Prep kit

• Indexing FAQ for Illumina DNA PCR-Free libraries

• Information about the TruSeq Index Plate Fixture

• Information and protocol for the Illumina 16S Metagenomics Sequencing Library Preparation workflow

• Information on the Illumina DNA/RNA UD Indexes

• Input recommendations FAQ for the TruPath Genome assay

• Input recommendations for Illumina Rapid RNA Kits

• Is the Illumina Tagment DNA (Nextera) enzyme, TDE1, pre-loaded with oligos?

• Kits, consumables, and reagent stability FAQ for Illumina Rapid RNA Kits

• Product capabilities FAQ for the TruPath Genome assay

• QC steps FAQ for Illumina Rapid RNA Kits

• Sequencing and run setup FAQ for Illumina Rapid RNA Kits

• Should indexes be read when sequencing a single library?

• Single or double-sided bead clean ups with Illumina DNA PCR-Free

• Viral Surveillance Panel (VSP) reagents and workflow frequently asked questions (FAQ)

• What are the concentrations of Illumina adapters and primers?

• What are the volume and number of enrichment reactions for Illumina Exome Panel (CEX)?

• What do (S), (M), and (L) mean in Illumina library prep kit names?

• What is the difference between a single- and dual-indexed library?

• What is the minimum library size that can be sequenced?

• Where are the sequences and plate positions of Illumina indexes?

• Workflow FAQ for Illumina Rapid RNA Kits

• Workflow and Sequencing recommendations FAQ for TruPath Genome