# FAQ

Library Preparation > General > FAQ

* [Can Illumina RNA Prep with Enrichment library prep and enrichment reagents be purchased separately?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006623)
* [Can PhiX and denatured PhiX be stored and used again?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005128)
* [Can RSB be used as a substitute for RSB + Tween 20 when sequencing on the NextSeq 1000/2000?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005890)
* [Can library preparation reagents be stored in a frost free freezer?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005798)
* [Can samples be sent to Illumina for sequencing; does Illumina offer sequencing as a service?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005495)
* [Can wells in index plates be used more than once?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005240)
* [Catalog/Reference numbers for IDT for Illumina DNA/RNA UD and Illumina DNA/RNA UD Indexes](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000008026)
* [Denature and dilution FAQ for Illumina DNA PCR Free](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000003444)
* [Does library loading differ between MiSeq Micro and Nano flow cells?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005606)
* [How much PhiX to spike in for well balanced, base diverse libraries?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006972)
* [How to perform library heat denaturation (heat shock) before sequencing?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005129)
* [How to prepare < 2 nM libraries for sequencing on NovaSeq X/X Plus](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000009000)
* [Hybridization Buffer (HT1) and Resuspension Buffer (RSB) alternatives and purchasing availability](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005040)
* [IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000001878)
* [Indexed Sequencing workflows with 5' to 3' directionality](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005679)
* [Information about the TruSeq index adapter plate fixture](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005724)
* [Introducing the Illumina DNA/RNA UD Indexes](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000008384)
* [Link to the Sequencing Methods Explorer](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006080)
* [Plate layout for the Illumina DNA/RNA UD Indexes (v3)](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000008364)
* [Product page and support page links for Illumina DNA Prep with Enrichment (S) Tagmentation](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005054)
* [Product page and support page links for Illumina RNA Prep with Enrichment, (L) Tagmentation](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005216)
* [Product page and support page links for TruSeq DNA Exome](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005343)
* [Product page and support page links for TruSeq Methyl Capture EPIC](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005242)
* [Product page and support page links for TruSight Cancer bundled with TruSight Rapid Capture](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005345)
* [Product page and support page links for TruSight Myeloid](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005346)
* [Sequences and plate positions for the Illumina Unique Dual Indexes, LT (48 Indexes, 48 Samples)](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000008386)
* [Should index reads be performed if only one library is being sequenced?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006541)
* [Should indexes be read when sequencing a single library?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006091)
* [What are the Illumina sequencing primer sequences?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000007129)
* [What do (S), (M), and (L) mean in Illumina library prep kit names?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006971)
* [What is a Single Read (SR), Paired End (PE) reads, and read requirements for sequencing?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000009547)
* [What is the concentration of Illumina adapters and primers?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005459)
* [What is the difference between a single and dual indexed library?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005669)
* [What is the minimum library size that can be sequenced?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006540)
* [Where are the sequences and plate positions of Illumina indexes?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000007378)
* [Where to find the reference sequence used for phiX alignment?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005582)
* [Which TruSeq index kits come in plate or tube format?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000002332)
* [Which computer operating systems are compatible with Illumina Experiment Manager (IEM)?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005722)