FAQ

Library Preparation > General > FAQ

• Can Illumina RNA Prep with Enrichment library prep and enrichment reagents be purchased separately?

• Can PhiX and denatured PhiX be stored and used again?

• Can RSB be used as a substitute for RSB + Tween 20 when sequencing on the NextSeq 1000/2000?

• Can library preparation reagents be stored in a frost free freezer?

• Can samples be sent to Illumina for sequencing; does Illumina offer sequencing as a service?

• Can wells in index plates be used more than once?

• Catalog/Reference numbers for IDT for Illumina DNA/RNA UD and Illumina DNA/RNA UD Indexes

• Denature and dilution FAQ for Illumina DNA PCR Free

• Does library loading differ between MiSeq Micro and Nano flow cells?

• How much PhiX to spike in for well balanced, base diverse libraries?

• How to perform library heat denaturation (heat shock) before sequencing?

• How to prepare < 2 nM libraries for sequencing on NovaSeq X/X Plus

• Hybridization Buffer (HT1) and Resuspension Buffer (RSB) alternatives and purchasing availability

• IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage

• Indexed Sequencing workflows with 5' to 3' directionality

• Information about the TruSeq index adapter plate fixture

• Introducing the Illumina DNA/RNA UD Indexes

• Link to the Sequencing Methods Explorer

• Plate layout for the Illumina DNA/RNA UD Indexes (v3)

• Product page and support page links for Illumina DNA Prep with Enrichment (S) Tagmentation

• Product page and support page links for Illumina RNA Prep with Enrichment, (L) Tagmentation

• Product page and support page links for TruSeq DNA Exome

• Product page and support page links for TruSeq Methyl Capture EPIC

• Product page and support page links for TruSight Cancer bundled with TruSight Rapid Capture

• Product page and support page links for TruSight Myeloid

• Should index reads be performed if only one library is being sequenced?

• Should indexes be read when sequencing a single library?

• What are the Illumina sequencing primer sequences?

• What are the index sequences for the AmpliSeq CD Indexes Large Volume kit for Illumina?

• What do (S), (M), and (L) mean in Illumina library prep kit names?

• What is a Single Read (SR), Paired End (PE) reads, and read requirements for sequencing?

• What is the concentration of Illumina adapters and primers?

• What is the difference between a single and dual indexed library?

• What is the minimum library size that can be sequenced?

• Where are the sequences and plate positions of Illumina indexes?

• Where to find the reference sequence used for phiX alignment?

• Which TruSeq index kits come in plate or tube format?

• Which computer operating systems are compatible with Illumina Experiment Manager (IEM)?