# FAQ

Library Preparation > General > FAQ

* [Can Illumina RNA Prep with Enrichment library prep and enrichment reagents be purchased separately?](/library-preparation/general/library-preparation-general-faq-list/000006623.md)
* [Can PhiX and denatured PhiX be stored and used again?](/library-preparation/general/library-preparation-general-faq-list/000005128.md)
* [Can RSB be used as a substitute for RSB + Tween 20 when sequencing on the NextSeq 1000/2000?](/library-preparation/general/library-preparation-general-faq-list/000005890.md)
* [Can library preparation reagents be stored in a frost free freezer?](/library-preparation/general/library-preparation-general-faq-list/000005798.md)
* [Can samples be sent to Illumina for sequencing; does Illumina offer sequencing as a service?](/library-preparation/general/library-preparation-general-faq-list/000005495.md)
* [Can wells in index plates be used more than once?](/library-preparation/general/library-preparation-general-faq-list/000005240.md)
* [Catalog/Reference numbers for IDT for Illumina DNA/RNA UD and Illumina DNA/RNA UD Indexes](/library-preparation/general/library-preparation-general-faq-list/000008026.md)
* [Denature and dilution FAQ for Illumina DNA PCR Free](/library-preparation/general/library-preparation-general-faq-list/000003444.md)
* [Does library loading differ between MiSeq Micro and Nano flow cells?](/library-preparation/general/library-preparation-general-faq-list/000005606.md)
* [How much PhiX to spike in for well balanced, base diverse libraries?](/library-preparation/general/library-preparation-general-faq-list/000006972.md)
* [How to perform library heat denaturation (heat shock) before sequencing?](/library-preparation/general/library-preparation-general-faq-list/000005129.md)
* [How to prepare < 2 nM libraries for sequencing on NovaSeq X/X Plus](/library-preparation/general/library-preparation-general-faq-list/000009000.md)
* [Hybridization Buffer (HT1) and Resuspension Buffer (RSB) alternatives and purchasing availability](/library-preparation/general/library-preparation-general-faq-list/000005040.md)
* [IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage](/library-preparation/general/library-preparation-general-faq-list/000001878.md)
* [Indexed Sequencing workflows with 5' to 3' directionality](/library-preparation/general/library-preparation-general-faq-list/000005679.md)
* [Information about the TruSeq index adapter plate fixture](/library-preparation/general/library-preparation-general-faq-list/000005724.md)
* [Introducing the Illumina DNA/RNA UD Indexes](/library-preparation/general/library-preparation-general-faq-list/000008384.md)
* [Link to the Sequencing Methods Explorer](/library-preparation/general/library-preparation-general-faq-list/000006080.md)
* [Plate layout for the Illumina DNA/RNA UD Indexes (v3)](/library-preparation/general/library-preparation-general-faq-list/000008364.md)
* [Product page and support page links for Illumina DNA Prep with Enrichment (S) Tagmentation](/library-preparation/general/library-preparation-general-faq-list/000005054.md)
* [Product page and support page links for Illumina RNA Prep with Enrichment, (L) Tagmentation](/library-preparation/general/library-preparation-general-faq-list/000005216.md)
* [Product page and support page links for TruSeq DNA Exome](/library-preparation/general/library-preparation-general-faq-list/000005343.md)
* [Product page and support page links for TruSeq Methyl Capture EPIC](/library-preparation/general/library-preparation-general-faq-list/000005242.md)
* [Product page and support page links for TruSight Cancer bundled with TruSight Rapid Capture](/library-preparation/general/library-preparation-general-faq-list/000005345.md)
* [Product page and support page links for TruSight Myeloid](/library-preparation/general/library-preparation-general-faq-list/000005346.md)
* [Sequences and plate positions for the Illumina Unique Dual Indexes, LT (48 Indexes, 48 Samples)](/library-preparation/general/library-preparation-general-faq-list/000008386.md)
* [Should index reads be performed if only one library is being sequenced?](/library-preparation/general/library-preparation-general-faq-list/000006541.md)
* [Should indexes be read when sequencing a single library?](/library-preparation/general/library-preparation-general-faq-list/000006091.md)
* [What are the Illumina sequencing primer sequences?](/library-preparation/general/library-preparation-general-faq-list/000007129.md)
* [What do (S), (M), and (L) mean in Illumina library prep kit names?](/library-preparation/general/library-preparation-general-faq-list/000006971.md)
* [What is a Single Read (SR), Paired End (PE) reads, and read requirements for sequencing?](/library-preparation/general/library-preparation-general-faq-list/000009547.md)
* [What is the concentration of Illumina adapters and primers?](/library-preparation/general/library-preparation-general-faq-list/000005459.md)
* [What is the difference between a single and dual indexed library?](/library-preparation/general/library-preparation-general-faq-list/000005669.md)
* [What is the minimum library size that can be sequenced?](/library-preparation/general/library-preparation-general-faq-list/000006540.md)
* [Where are the sequences and plate positions of Illumina indexes?](/library-preparation/general/library-preparation-general-faq-list/000007378.md)
* [Where to find the reference sequence used for phiX alignment?](/library-preparation/general/library-preparation-general-faq-list/000005582.md)
* [Which TruSeq index kits come in plate or tube format?](/library-preparation/general/library-preparation-general-faq-list/000002332.md)
* [Which computer operating systems are compatible with Illumina Experiment Manager (IEM)?](/library-preparation/general/library-preparation-general-faq-list/000005722.md)


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